Cloned (Comment) | Organism |
---|---|
expression in Saccharomyces cerevisiae | Sporothrix schenckii |
Protein Variants | Comment | Organism |
---|---|---|
biotechnology | the recombinant enzyme expressed in Saccharomyces cereviaise reveals some differences from the wild type enzyme, such as improved stability and less sensitivity to UDP-GlcNAc | Sporothrix schenckii |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-amino-2-deoxy-D-mannitol 6-phosphate | - |
Sporothrix schenckii | |
N3-(4-Methoxyfumaroyl)-L-2,3-diaminopropanoic acid | selective | Sporothrix schenckii | |
UDP-N-acetylglucosamine | maximum inhibition at 1 mM | Sporothrix schenckii |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
79000 | - |
x * 79000, SDS-PAGE | Sporothrix schenckii |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sporothrix schenckii | D3YIG6 | - |
- |
Storage Stability | Organism |
---|---|
-20°C, potassium phosphate buffer, pH 7.0, containing 1 mM EDTA, 1 mM DTT, 50% glycerol and 10 mM fructose 6-phosphate, fully stable | Sporothrix schenckii |
4°C, potassium phosphate buffer, pH 7.0, containing 1 mM EDTA, 1 mM DTT, 50% glycerol and 10 mM fructose 6-phosphate, complete loss of activity within 24 h | Sporothrix schenckii |
Subunits | Comment | Organism |
---|---|---|
? | x * 79000, SDS-PAGE | Sporothrix schenckii |
Synonyms | Comment | Organism |
---|---|---|
Gfa1 | - |
Sporothrix schenckii |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.013 | - |
pH not specified in the publication, temperature not specified in the publication | Sporothrix schenckii | N3-(4-Methoxyfumaroyl)-L-2,3-diaminopropanoic acid | |
0.073 | - |
pH not specified in the publication, temperature not specified in the publication | Sporothrix schenckii | 2-amino-2-deoxy-D-mannitol 6-phosphate | |
0.45 | - |
crude enzyme preparation, pH not specified in the publication, temperature not specified in the publication | Sporothrix schenckii | UDP-N-acetylglucosamine |
General Information | Comment | Organism |
---|---|---|
physiological function | the recombinant enzyme restores glucosamine prototrophy of the Saccharomyces cerevisiae gfa1 null mutant | Sporothrix schenckii |