Literature summary for 2.6.1.16 extracted from

Gaucher-Wieczorek, F.; Guerineau, V.; Touboul, D.; Thetiot-Laurent, S.; Pelissier, F.; Badet-Denisot, M.A.; Badet, B.; Durand, P.
Evaluation of synthase and hemisynthase activities of glucosamine-6-phosphate synthase by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (2014), Anal. Biochem., 458, 61-65.

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Application

Application	Comment	Organism
analysis	assay of synthase and hemisynthase activities of glucosamine-6-phosphate synthase by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry, allowing determination of both hemisynthase and synthase parameters from a single assay kinetic experiment	Escherichia coli

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.11	-	L-glutamine	synthase activity, pH 7.2, 37°C	Escherichia coli
0.14	-	L-glutamine	hemisynthase activity, pH 7.2, 37°C	Escherichia coli
0.5	-	D-fructose 6-phosphate	synthase activity, pH 7.2, 37°C	Escherichia coli
0.8	-	D-fructose 6-phosphate	hemisynthase activity, pH 7.2, 37°C	Escherichia coli

Organism

Organism	UniProt	Comment	Textmining
Escherichia coli	P17169	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
L-glutamine + D-fructose 6-phosphate	-	Escherichia coli	L-glutamate + D-glucosamine 6-phosphate	-	?
additional information	enzyme follows an ordered process to bind sequentially Fru-6P and L-Gln and successively releases L-glutamate (hemisynthase activity) and D-glucosamine 6-phosphate (synthase activity)	Escherichia coli	?	-	?

Synonyms

Synonyms	Comment	Organism
GlmS	-	Escherichia coli

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
16.4	-	D-fructose 6-phosphate	hemisynthase activity, pH 7.2, 37°C	Escherichia coli
16.8	-	L-glutamine	hemisynthase activity, pH 7.2, 37°C	Escherichia coli
16.9	-	D-fructose 6-phosphate	synthase activity, pH 7.2, 37°C	Escherichia coli
16.95	-	L-glutamine	synthase activity, pH 7.2, 37°C	Escherichia coli

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Release 2023.1 (January 2023)