Protein Variants | Comment | Organism |
---|---|---|
C1A | C1A-GlmS does not reveal glutaminase activity at 37°C when tested in the presence of Gln only | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.2 | - |
L-glutamate | calorimetric determination, pH 7.2, 25°C | Escherichia coli | |
0.23 | - |
D-fructose 6-phosphate | calorimetric determination, pH 7.2, 37°C | Escherichia coli | |
0.3 | - |
D-fructose 6-phosphate | calorimetric determination, pH 7.2, 25°C | Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
66640 | - |
SDS-PAGE | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-glutamine + D-fructose 6-phosphate | - |
Escherichia coli | L-glutamate + D-glucosamine 6-phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | sedimentation velocity experiments show that at low concentration the enzyme is mainly present as a dimer. At a higher protein concentration the equilibrium between the two forms of GlmS is significantly displaced toward the oligomeric form | Escherichia coli |
oligomer | sedimentation velocity experiments show that at low concentration the enzyme is mainly present as a dimer. At a higher protein concentration the equilibrium between the two forms of GlmS is significantly displaced toward the oligomeric form | Escherichia coli |
Synonyms | Comment | Organism |
---|---|---|
GlmS | in the absence of D-fructose 6-phosphate, the enzyme exhibits a weak hydrolyzing activity of Gln into Glu and ammonia (glutaminase activity), whereas the presence of D-fructose 6-phosphate strongly stimulates its hemisynthase activity | Escherichia coli |
glucosamine-6-phosphate synthase | - |
Escherichia coli |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Escherichia coli |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
9.81 | - |
L-glutamate | calorimetric determination, pH 7.2, 25°C | Escherichia coli | |
9.9 | - |
D-fructose 6-phosphate | calorimetric determination, pH 7.2, 25°C | Escherichia coli | |
12 | - |
D-fructose 6-phosphate | calorimetric determination, pH 7.2, 37°C | Escherichia coli |