Cloned (Comment) | Organism |
---|---|
expression in insect cell and Escherichia coli | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
S243E | increase in activity, 2fold lower Km value for D-fructose 6-phosphate than wild-type | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.52 | - |
D-fructose 6-phosphate | mutant S243E, 37°C, pH 7.5 | Homo sapiens | |
1.04 | - |
D-fructose 6-phosphate | wild-type, 37°C, pH 7.5 | Homo sapiens | |
1.8 | - |
L-gamma-glutamyl-p-nitroanilide | both wild-type and mutant S243E, 37°C, pH 7.5 | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
glutamine:fructose-6-phosphate amidotransferase isoform 1 | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
phosphoprotein | enzyme is phosphorylated in vivo, phosphorylation site is residue S243. Phosphorylation at Ser243 stimulates glucosamine 6-phosphate synthesizing activity, lowers amidohydrolyzing activity in the absence of fructose 6-phosphate, and lowers Km of fructose 6-phosphate 2fold, but has no effect on UDP-GlcNAc inhibition. Phosphorylation is mediated by AMP-activated protein kinase and calcium/calmodulin-dependent kinase II in vitro | Homo sapiens |
Storage Stability | Organism |
---|---|
-80°C, 50 mM Tris-HCl, pH 7.8, 200 mM NaCl, 1 mM fructose 6-phosphate, 2 mM tris(2-carboxyethyl)phosphine hydrochloride, 200 mM imidazole, 10% glycerol, stable for up to 12 months | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-gamma-glutamyl-p-nitroanilide + H2O | determination of amidohydrolysing activity | Homo sapiens | L-glutamine + p-nitroaniline | - |
? | |
L-glutamine + D-fructose 6-phosphate | - |
Homo sapiens | L-glutamate + D-glucosamine 6-phosphate | - |
? |