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Literature summary for 2.6.1.16 extracted from
- Characterisation of glutamine fructose-6-phosphate amidotransferase (EC 2.6.1.16) and N-acetylglucosamine metabolism in Bifidobacterium (2008), Arch. Microbiol., 189, 157-167.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression as His-tagged protein | Bifidobacterium longum |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 5.1 | - |
L-glutamine | pH 7.0, 55°C | Bifidobacterium longum |  |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 68600 | - |
x * 68600, calculated for native protein, x * 69600, calculated, and x * 69400, SDS-PAGE, His-tagged protein, respectively | Bifidobacterium longum |
| 69400 | - |
x * 68600, calculated for native protein, x * 69600, calculated, and x * 69400, SDS-PAGE, His-tagged protein, respectively | Bifidobacterium longum |
| 69600 | - |
x * 68600, calculated for native protein, x * 69600, calculated, and x * 69400, SDS-PAGE, His-tagged protein, respectively | Bifidobacterium longum |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bifidobacterium longum | Q8G545 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant protein | Bifidobacterium longum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-glutamine + D-fructose 6-phosphate | - |
Bifidobacterium longum | L-glutamate + D-glucosamine 6-phosphate | - |
? | |
| additional information | enzyme possesses an independent glutaminase activity, converting glutamine to glutamate in the absence of fructose 6-phosphate | Bifidobacterium longum | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 68600, calculated for native protein, x * 69600, calculated, and x * 69400, SDS-PAGE, His-tagged protein, respectively | Bifidobacterium longum |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 55 | - |
- |
Bifidobacterium longum |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
- |
Bifidobacterium longum |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 8 | marked decrease in activity at pH values below 6.0 and above 8.0 | Bifidobacterium longum |
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