Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) pLysS cells | Candida albicans |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.2 | - |
D-fructose 6-phosphate | wild type enzyme, in in 25 mM potassium phosphate buffer at pH 7.0 and 37°C | Candida albicans |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
79140 | - |
Superdex 200 HR 10/30 gel filtration | Candida albicans |
80000 | - |
4 * 80000, SDS-PAGE | Candida albicans |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Candida albicans | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Ni2+-IDA agarose chromatography | Candida albicans |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.631 | - |
wild type enzyme, using D-fructose 6-phosphate as a substrate | Candida albicans |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-glutamine + D-fructose 6-phosphate | - |
Candida albicans | L-glutamate + D-glucosamine 6-phosphate | - |
ir |
Subunits | Comment | Organism |
---|---|---|
homotetramer | 4 * 80000, SDS-PAGE | Candida albicans |
Synonyms | Comment | Organism |
---|---|---|
GlcN-6-P synthase | - |
Candida albicans |
glucosamine-6-phosphate synthase | - |
Candida albicans |
L-glutamine: D-fructose-6-phosphate amidotransferase | - |
Candida albicans |