Activating Compound | Comment | Organism | Structure |
---|---|---|---|
dithiothreitol | 1 mM, activates 1.8fold | Saccharomyces cerevisiae |
General Stability | Organism |
---|---|
complete loss of activity after treatment with ammonium sulfate, 30-50% saturation | Saccharomyces cerevisiae |
dithiothreitol, D-fructose 6-phosphate, D-glucose 6-phosphate or L-glutamine, 1 mM, increases thermal stability at 37°C | Saccharomyces cerevisiae |
freezing and thawing has no effect on activity | Saccharomyces cerevisiae |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Mercuric chloride | 84% inhibition at 1 mM | Saccharomyces cerevisiae | |
N-ethylmaleimide | 78% inhibition at1 mM | Saccharomyces cerevisiae | |
p-chloromercuribenzoate | 84% inhibition at 0.1 mM | Saccharomyces cerevisiae | |
UDP-N-acetylglucosamine | 40% inhibition at 1 mM | Saccharomyces cerevisiae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-glutamine + D-fructose 6-phosphate | Saccharomyces cerevisiae | - |
L-glutamate + D-glucosamine 6-phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | - |
- |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.00783 | - |
- |
Saccharomyces cerevisiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-glutamine + D-fructose 6-phosphate | - |
Saccharomyces cerevisiae | L-glutamate + D-glucosamine 6-phosphate | - |
? | |
L-glutamine + D-fructose 6-phosphate | specific for: L-glutamine | Saccharomyces cerevisiae | L-glutamate + D-glucosamine 6-phosphate | - |
? |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
90 min, complete loss of activity without stabilizing agent. Dithiothreitol, D-fructose 6-phosphate, D-glucose 6-phosphate or L-glutamine, 1 mM, increase thermal stability at 37°C | Saccharomyces cerevisiae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
potassium phosphate buffer | Saccharomyces cerevisiae |