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Literature summary for 2.6.1.113 extracted from

  • Yonaha, K.; Toyama, S.
    Diamine transamination catalyzed by omega-amino acid:pyruvate aminotransferase of Pseudomonas sp. f-126 (1979), Agric. Biol. Chem., 43, 1043-1048.
No PubMed abstract available
Search for more literature for 2.6.1.113

Activating Compound

Activating Compound Comment Organism Structure
pyridoxal 5'-phosphate preincubation with pyridoxal phosphate leads to an increase in the enzyme activity and the protection of the activity against heat inactivation at 40-60 and 65-80°C, respectively Pseudomonas sp.

Inhibitors

Inhibitors Comment Organism Structure
3-methyl-2-benzothiazolone hydrazone
-
 Pseudomonas sp.
aminoxy acetate
-
 Pseudomonas sp.
D-cycloserine
-
 Pseudomonas sp.
hydroxylamine
-
 Pseudomonas sp.
phenylhydrazine
-
 Pseudomonas sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00625
-
 pyruvate pH 10.0, temperature not specified in the publication Pseudomonas sp.
0.0769
-
 putrescine pH 10.0, temperature not specified in the publication Pseudomonas sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1,2-diaminoethane + pyruvate Pseudomonas sp. 38% of the activity with beta-alanine aminoethane + alanine
-
 ?
1,2-diaminoethane + pyruvate Pseudomonas sp. F-126 38% of the activity with beta-alanine aminoethane + alanine
-
 ?
1,6-diaminohexane + pyruvate Pseudomonas sp. 87% of the activity with beta-alanine 1-aminohexane + alanine
-
 ?
1,6-diaminohexane + pyruvate Pseudomonas sp. F-126 87% of the activity with beta-alanine 1-aminohexane + alanine
-
 ?
1,7-diaminoheptane + pyruvate Pseudomonas sp. 92% of the activity with beta-alanine 1-aminoheptane + alanine
-
 ?
1,7-diaminoheptane + pyruvate Pseudomonas sp. F-126 92% of the activity with beta-alanine 1-aminoheptane + alanine
-
 ?
1,8-diaminooctane + pyruvate Pseudomonas sp. 55% of the activity with beta-alanine 1-aminooctane + alanine
-
 ?
1,8-diaminooctane + pyruvate Pseudomonas sp. F-126 55% of the activity with beta-alanine 1-aminooctane + alanine
-
 ?
beta-alanine + pyruvate Pseudomonas sp. best substrate 3-oxopropionate + alanine
-
 ?
cadaverine + pyruvate Pseudomonas sp. 12% of the activity with beta-alanine 1-aminopentane + alanine
-
 ?
additional information Pseudomonas sp. one of the two amino groups of 1,2-diaminoethane, putrescine and cadaverine transaminates to pyruvate. The transamination between putrescine and pyruvate proceeds by a ping-pong bi bi mechanism. No substrate: 1,3-diaminopropane ?
-
 ?
additional information Pseudomonas sp. F-126 one of the two amino groups of 1,2-diaminoethane, putrescine and cadaverine transaminates to pyruvate. The transamination between putrescine and pyruvate proceeds by a ping-pong bi bi mechanism. No substrate: 1,3-diaminopropane ?
-
 ?
putrescine + pyruvate Pseudomonas sp. 55% of the activity with beta-alanine 4-aminobutanal + alanine
-
 ?

Organism

Organism UniProt Comment Textmining
Pseudomonas sp.
-
-
-
Pseudomonas sp. F-126
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Pseudomonas sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1,2-diaminoethane + pyruvate 38% of the activity with beta-alanine Pseudomonas sp. aminoethane + alanine
-
 ?
1,2-diaminoethane + pyruvate 38% of the activity with beta-alanine Pseudomonas sp. F-126 aminoethane + alanine
-
 ?
1,6-diaminohexane + pyruvate 87% of the activity with beta-alanine Pseudomonas sp. 1-aminohexane + alanine
-
 ?
1,6-diaminohexane + pyruvate 87% of the activity with beta-alanine Pseudomonas sp. F-126 1-aminohexane + alanine
-
 ?
1,7-diaminoheptane + pyruvate 92% of the activity with beta-alanine Pseudomonas sp. 1-aminoheptane + alanine
-
 ?
1,7-diaminoheptane + pyruvate 92% of the activity with beta-alanine Pseudomonas sp. F-126 1-aminoheptane + alanine
-
 ?
1,8-diaminooctane + pyruvate 55% of the activity with beta-alanine Pseudomonas sp. 1-aminooctane + alanine
-
 ?
1,8-diaminooctane + pyruvate 55% of the activity with beta-alanine Pseudomonas sp. F-126 1-aminooctane + alanine
-
 ?
beta-alanine + pyruvate best substrate Pseudomonas sp. 3-oxopropionate + alanine
-
 ?
cadaverine + pyruvate 12% of the activity with beta-alanine Pseudomonas sp. 1-aminopentane + alanine
-
 ?
additional information one of the two amino groups of 1,2-diaminoethane, putrescine and cadaverine transaminates to pyruvate. The transamination between putrescine and pyruvate proceeds by a ping-pong bi bi mechanism. No substrate: 1,3-diaminopropane Pseudomonas sp. ?
-
 ?
additional information one of the two amino groups of 1,2-diaminoethane, putrescine and cadaverine transaminates to pyruvate. The transamination between putrescine and pyruvate proceeds by a ping-pong bi bi mechanism. No substrate: 1,3-diaminopropane Pseudomonas sp. F-126 ?
-
 ?
putrescine + pyruvate 55% of the activity with beta-alanine Pseudomonas sp. 4-aminobutanal + alanine
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
-
 Pseudomonas sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60 65 in presence of pyridoxal 5'-phosphate, inactivation is delayed Pseudomonas sp.
70
-
 rapid inactivation above Pseudomonas sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
10
-
 assay at Pseudomonas sp.
10.5 11
-
 Pseudomonas sp.

pH Stability

pH Stability pH Stability Maximum Comment Organism
8 10 stable within Pseudomonas sp.

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate
-
 Pseudomonas sp.

Expression

Organism Comment Expression
Pseudomonas sp. expression is induced in presence of beta-alanine up