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Literature summary for 2.6.1.100 extracted from
- Biosynthesis of aminoglycoside antibiotics: cloning, expression and characterisation of an aminotransferase involved in the pathway to 2-deoxystreptamine (2002), Chem. Commun. (Camb. ), 2002, 2860-2861.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene btrR, recombinant expression of His-tagged BtrR in Escherichia coli strain BL 21 (DE3) | Niallia circulans |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 50000 | - |
2 * 50000, recombinant His-tagged enzyme, SDS-PAGE, 2 * 50714, mass spectrometry, 2 * 50703, sequence calculation | Niallia circulans |
| 50703 | - |
2 * 50000, recombinant His-tagged enzyme, SDS-PAGE, 2 * 50714, mass spectrometry, 2 * 50703, sequence calculation | Niallia circulans |
| 50714 | - |
2 * 50000, recombinant His-tagged enzyme, SDS-PAGE, 2 * 50714, mass spectrometry, 2 * 50703, sequence calculation | Niallia circulans |
| 97000 | - |
recombinant His-tagged enzyme, gel filtration | Niallia circulans |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-glutamine + 2-deoxy-scyllo-inosose | Niallia circulans | - |
2-oxoglutaramate + 2-deoxy-scyllo-inosamine | - |
? |  |
| L-glutamine + 2-deoxy-scyllo-inosose | Niallia circulans NRRL B3312 | - |
2-oxoglutaramate + 2-deoxy-scyllo-inosamine | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Niallia circulans | Q8G8Y2 | gene btrR encoded in the butirosin cluster | - |
| Niallia circulans NRRL B3312 | Q8G8Y2 | gene btrR encoded in the butirosin cluster | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His-tagged BtrR from Escherichia coli strain BL 21 (DE3) by cobalt affinity chromatography, gel filtration, and anion exchange chromatography | Niallia circulans |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-glutamine + 2-deoxy-scyllo-inosose | - |
Niallia circulans | 2-oxoglutaramate + 2-deoxy-scyllo-inosamine | - |
? | |
| L-glutamine + 2-deoxy-scyllo-inosose | - |
Niallia circulans NRRL B3312 | 2-oxoglutaramate + 2-deoxy-scyllo-inosamine | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| homodimer | 2 * 50000, recombinant His-tagged enzyme, SDS-PAGE, 2 * 50714, mass spectrometry, 2 * 50703, sequence calculation | Niallia circulans |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| btrR | - |
Niallia circulans |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Niallia circulans |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
assay at | Niallia circulans |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| pyridoxal 5'-phosphate | - |
Niallia circulans | |
General Information
| General Information | Comment | Organism |
|---|---|---|
| metabolism | the enzyme catalyses the transamination of 2-deoxy-scyllo-inosose to give 2-deoxy-scyllo-inosamine, an intermediate in the biosynthesis of 2-deoxystreptamine, biosynthetic pathway of 2-deoxystreptamine, overview | Niallia circulans |
| physiological function | BtrR catalyzes the synthesis of 2-deoxystreptamine, which is the common aglycone of a class of aminoglycoside antibiotics, of which many are clinically important aminoglycosides like kanamycin, neomycin, gentamicin and butirosin | Niallia circulans |
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