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Literature summary for 2.6.1.100 extracted from

  • Huang, F.; Haydock, S.F.; Mironenko, T.; Spiteller, D.; Li, Y.; Spencer, J.B.
    The neomycin biosynthetic gene cluster of Streptomyces fradiae NCIMB 8233: characterization of an aminotransferase involved in the formation of 2-deoxystreptamine (2005), Org. Biomol. Chem., 3, 1410-1418.
    View publication on PubMed
Search for more literature for 2.6.1.100

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-glutamine + 2-deoxy-scyllo-inosose Streptomyces fradiae
-
 2-oxoglutaramate + 2-deoxy-scyllo-inosamine
-
 ?
L-glutamine + 2-deoxy-scyllo-inosose Streptomyces fradiae NCIMB 8233
-
 2-oxoglutaramate + 2-deoxy-scyllo-inosamine
-
 ?
additional information Streptomyces fradiae purified Neo-6 also catalyses the amino transfer from L-glutamine to 3-amino-2,3-dideoxy-scyllo-inosose to produce 2-deoxystrepamine, EC 2.6.1.101 ?
-
 ?
additional information Streptomyces fradiae NCIMB 8233 purified Neo-6 also catalyses the amino transfer from L-glutamine to 3-amino-2,3-dideoxy-scyllo-inosose to produce 2-deoxystrepamine, EC 2.6.1.101 ?
-
 ?

Organism

Organism UniProt Comment Textmining
Streptomyces fradiae
-
-
-
Streptomyces fradiae
-
 gene tbmBr
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Streptomyces fradiae NCIMB 8233
-
-
-
Streptomyces fradiae NCIMB 8233
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 gene tbmBr
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-glutamine + 2-deoxy-scyllo-inosose
-
 Streptomyces fradiae 2-oxoglutaramate + 2-deoxy-scyllo-inosamine
-
 ?
L-glutamine + 2-deoxy-scyllo-inosose
-
 Streptomyces fradiae NCIMB 8233 2-oxoglutaramate + 2-deoxy-scyllo-inosamine
-
 ?
additional information purified Neo-6 also catalyses the amino transfer from L-glutamine to 3-amino-2,3-dideoxy-scyllo-inosose to produce 2-deoxystrepamine, EC 2.6.1.101 Streptomyces fradiae ?
-
 ?
additional information purified Neo-6 also catalyses the amino transfer from L-glutamine to 3-amino-2,3-dideoxy-scyllo-inosose to produce 2-deoxystrepamine, EC 2.6.1.101 Streptomyces fradiae NCIMB 8233 ?
-
 ?

Synonyms

Synonyms Comment Organism
btrR
-
 Streptomyces fradiae
BtrS
-
 Streptomyces fradiae
kanB
-
 Streptomyces fradiae
neo-6
-
 Streptomyces fradiae
neoB
-
 Streptomyces fradiae

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Streptomyces fradiae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 assay at Streptomyces fradiae

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate the BtrR-bound pyridoxal 5'-phosphate is the essential cofactor for the amino-transfer activity Streptomyces fradiae

General Information

General Information Comment Organism
metabolism Neo-6 is the enzyme catalysing the transamination of 2-deoxy-scyllo-inosose, DOI, using L-glutamine as an amino donor to produce 2-deoxyscyllo-inosamine as required for 2-deoxystreptamine biosynthesis. Neo-6 also catalyses the amino transfer from L-glutamine to 3-amino-2,3-dideoxy-scyllo-inosose to produce 2-deoxystrepamine, EC 2.6.1.101, the aglycon in neomycins, overview Streptomyces fradiae
additional information gene neo6 of the neo gene cluster and btrR from the btr gene cluster are equivalent, genetic organization, overview Streptomyces fradiae
physiological function the enzyme produces 2-deoxystreptamine (DOS), EC 2.6.1.101, which is the aglycone in neomycins, overview. Neo-6 is also the enzyme catalysing the transamination of 2-deoxy-scyllo-inosose, DOI, using L-glutamine as an amino donor to produce 2-deoxyscyllo-inosamine as required for 2-deoxystreptamine biosynthesis Streptomyces fradiae