Cloned (Comment) | Organism |
---|---|
overexpressed in Escherichia coli | Bubalus bubalis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.4 | - |
2-oxoglutarate | pH 7.5, 35°C | Bubalus bubalis | |
4.6 | - |
L-aspartate | pH 7.5, 35°C | Bubalus bubalis |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Bubalus bubalis | 5829 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
40000 | - |
SDS-PAGE | Bubalus bubalis |
46410 | - |
deduced from cDNA | Bubalus bubalis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bubalus bubalis | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
heart | - |
Bubalus bubalis | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-aspartate + 2-oxoglutarate | - |
Bubalus bubalis | oxaloacetate + L-glutamate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
aspartate aminotransferase | - |
Bubalus bubalis |
AST-Bb | - |
Bubalus bubalis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
highest activity | Bubalus bubalis |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
15 | - |
30% activity | Bubalus bubalis |
60 | - |
30% activity | Bubalus bubalis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
75 | - |
no significant decrease in the enzyme activity even after 30 min of incubation at 75°C | Bubalus bubalis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
352 | - |
L-aspartate | pH 7.5, 35°C | Bubalus bubalis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
highest activity | Bubalus bubalis |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | - |
40% of maximal activity | Bubalus bubalis |
10 | - |
40% of maximal activity | Bubalus bubalis |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
76.5 | - |
L-aspartate | pH 7.5, 35°C | Bubalus bubalis | |
251.4 | - |
2-oxoglutarate | pH 7.5, 35°C | Bubalus bubalis |