Literature summary for 2.6.1.1 extracted from

Mattingly, J.R., Jr.; Yanez, A.J.; Martinez-Carrion, M.
The folding of nascent mitochondrial aspartate aminotransferase synthesized in a cell-free extract can be assisted by GroEL and GroES (2000), Arch. Biochem. Biophys., 382, 113-122.

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Activating Compound

Activating Compound	Comment	Organism	Structure
additional information	intramitochondrial chaperone homologues GroEL and GroES can facilitate the folding of nascent premature mitochondrial isoform pmAspAT in rabbit reticulocyte lysate under conditions where it otherwise would not, GroEL alone inhibits the import into mitochondria, which is reversed by GroES	Rattus norvegicus

General Stability

General Stability	Organism
enzyme is stabilized by the intramitochondrial chaperone homologues GroEL and GroES	Rattus norvegicus

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
mitochondrion	mature mAspAT and premature pmAspAT forms of the isozyme	Rattus norvegicus	5739	-

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
47000	-	x * 47000, premature mitochondrial isozyme pmAspAT, SDS-PAGE	Rattus norvegicus

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
L-aspartate + 2-oxoglutarate	Rattus norvegicus	-	oxaloacetate + L-glutamate	-	?

Organism

Organism	UniProt	Comment	Textmining
Rattus norvegicus	-	mitochondrial isozyme mAspAT	-

Renatured (Commentary)

Renatured (Comment)	Organism
guanidinium hydrochloride denatured premature form pmAspAT cannot refold at 30°C, but refolds rapidly in presence of the intramitochondrial chaperone homologues GroEL and GroES	Rattus norvegicus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
L-aspartate + 2-oxoglutarate	-	Rattus norvegicus	oxaloacetate + L-glutamate	-	?

Subunits

Subunits	Comment	Organism
?	x * 47000, premature mitochondrial isozyme pmAspAT, SDS-PAGE	Rattus norvegicus

pI Value

Organism	Comment	pI Value Maximum	pI Value
Rattus norvegicus	pmAspAT	-	9.6

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Release 2023.1 (January 2023)