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Literature summary for 2.6.1.1 extracted from

  • Azzariti, A.; Vacca, R.A.; Giannattasio, S.; Merafina, R.S.; Marra, E.; Doonan, S.
    Kinetic properties and thermal stabilities of mutant forms of mitochondrial aspartate aminotransferase (1998), Biochim. Biophys. Acta, 1386, 29-38.
    View publication on PubMed
Search for more literature for 2.6.1.1

Cloned(Commentary)

Cloned (Comment) Organism
expression of wild-type mAspAT and pmAspAT and mutants in Escherichia coli Gallus gallus

Protein Variants

Protein Variants Comment Organism
C166A site-directed mutagenesis of isozyme mAspAT, decreased ability to undergo transition from the open to the closed conformation essential for the reaction mechanism, reduced reactivity with DTNB Gallus gallus
C166S site-directed mutagenesis of isozyme mAspAT, decreased ability to undergo transition from the open to the closed conformation essential for the reaction mechanism, reduced reactivity with DTNB Gallus gallus
additional information construction of deletion mutant DELTA3-11mAspAT of isozyme mAspAT, enhanced thermostability, reduced kcat and Km, enhanced reactivity of Cys166 with DTNB Gallus gallus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.007
-
 2-oxoglutarate recombinant deletion mutant, pH 7.5, 25°C Gallus gallus
0.08
-
 2-oxoglutarate recombinant mutant C166S, pH 7.5, 25°C Gallus gallus
0.09
-
 L-aspartate recombinant deletion mutant, pH 7.5, 25°C Gallus gallus
0.25
-
 L-aspartate recombinant premature isozyme pmAspAT, pH 7.5, 25°C Gallus gallus
0.29
-
 L-aspartate recombinant mutant C166A, pH 7.5, 25°C Gallus gallus
0.3
-
 2-oxoglutarate recombinant isozyme mAspAT, pH 7.5, 25°C Gallus gallus
0.33
-
 L-aspartate recombinant mutant C166S, pH 7.5, 25°C Gallus gallus
0.33
-
 2-oxoglutarate recombinant premature isozyme pmAspAT, pH 7.5, 25°C Gallus gallus
0.36
-
 L-aspartate recombinant isozyme mAspAT, pH 7.5, 25°C Gallus gallus
0.8
-
 2-oxoglutarate recombinant mutant C166A, pH 7.5, 25°C Gallus gallus

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol cytosolic isoenzyme Gallus gallus 5829
-
mitochondrion mature mAspAT and premature pmAspAT forms of the isozyme Gallus gallus 5739
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-aspartate + 2-oxoglutarate Gallus gallus
-
 oxaloacetate + L-glutamate
-
 ?

Organism

Organism UniProt Comment Textmining
Gallus gallus
-
 cytosolic and mitochondrial isozymes
-

Purification (Commentary)

Purification (Comment) Organism
recombinant mAspAT, pmAspAT and mutants from Escherichia coli Gallus gallus

Reaction

Reaction Comment Organism Reaction ID
L-aspartate + 2-oxoglutarate = oxaloacetate + L-glutamate bi bi ping pong reaction kinetic Gallus gallus
a

Source Tissue

Source Tissue Comment Organism Textmining
heart
-
 Gallus gallus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-aspartate + 2-oxoglutarate
-
 Gallus gallus oxaloacetate + L-glutamate
-
 ?

Synonyms

Synonyms Comment Organism
AspAT
-
 Gallus gallus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
 assay at Gallus gallus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
additional information
-
 the premature form of the mitochondrial isozyme is more thermostable than the mature form Gallus gallus
70
-
 t1/2 wild-type of mature mitochondrial isozyme: 2.7 min Gallus gallus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4
-
 2-oxoglutarate recombinant deletion mutant, pH 7.5, 25°C Gallus gallus
4
-
 L-aspartate recombinant deletion mutant, pH 7.5, 25°C Gallus gallus
89
-
 2-oxoglutarate recombinant C166S mutant, pH 7.5, 25°C Gallus gallus
89
-
 L-aspartate recombinant C166S mutant, pH 7.5, 25°C Gallus gallus
90
-
 2-oxoglutarate recombinant premature isozyme pmAspAT, pH 7.5, 25°C Gallus gallus
90
-
 L-aspartate recombinant premature isozyme pmAspAT, pH 7.5, 25°C Gallus gallus
96
-
 2-oxoglutarate recombinant C166A mutant, pH 7.5, 25°C Gallus gallus
96
-
 L-aspartate recombinant C166A mutant, pH 7.5, 25°C Gallus gallus
187
-
 2-oxoglutarate recombinant isozyme mAspAT, pH 7.5, 25°C Gallus gallus
187
-
 L-aspartate recombinant isozyme mAspAT, pH 7.5, 25°C Gallus gallus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 recombinant enzyme, assay at Gallus gallus