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Literature summary for 2.6.1.1 extracted from
- Crystallization and immunological identification of dog heart aspartate aminotransferase isoenzymes (1972), J. Biochem., 72, 511-519.
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
- |
Canis lupus familiaris |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.51 | - |
L-aspartate | mitochondrial isozyme | Canis lupus familiaris |  |
| 1.7 | - |
2-oxoglutarate | cytosolic isozyme | Canis lupus familiaris | |
| 2.2 | - |
2-oxoglutarate | mitochondrial isozyme | Canis lupus familiaris | |
| 6.5 | - |
L-aspartate | cytosolic isozyme | Canis lupus familiaris | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytosol | cytosolic isoenzyme | Canis lupus familiaris | 5829 | - |
| mitochondrion | mitochondrial isoenzyme | Canis lupus familiaris | 5739 | - |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-aspartate + 2-oxoglutarate | Canis lupus familiaris | - |
oxaloacetate + L-glutamate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Canis lupus familiaris | - |
mongrel dogs | - |
| Canis lupus familiaris | - |
cytosolic and mitochondrial isozymes | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| partial, cytosolic, 171fold, and mitochondrial, 78fold, isozymes | Canis lupus familiaris |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| heart | - |
Canis lupus familiaris | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 156 | - |
mitochondrial fraction | Canis lupus familiaris |
| 341 | - |
supernatant fraction | Canis lupus familiaris |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-aspartate + 2-oxoglutarate | - |
Canis lupus familiaris | oxaloacetate + L-glutamate | - |
? | |
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