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Literature summary for 2.6.1.1 extracted from

  • Muriana, F.J.G.; Alvarez-Ossorio, M.C.; Relimpio, A.M.
    Further thermal characterization of an aspartate aminotransferase from a halophilic organism (1994), Biochem. J., 298, 465-470.
No PubMed abstract available
Search for more literature for 2.6.1.1

General Stability

General Stability Organism
2-oxoglutarate stabilizes against thermal inactivation Haloferax mediterranei

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-aspartate + 2-oxoglutarate Haloferax mediterranei
-
 oxaloacetate + L-glutamate
-
 ?
L-aspartate + 2-oxoglutarate Haloferax mediterranei R-4 / ATCC 33500
-
 oxaloacetate + L-glutamate
-
 ?

Organism

Organism UniProt Comment Textmining
Haloferax mediterranei
-
-
-
Haloferax mediterranei R-4 / ATCC 33500
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Haloferax mediterranei

Renatured (Commentary)

Renatured (Comment) Organism
reconstitution of holoenzyme after purification of apoenzyme with pyridoxal 5'-phosphate Haloferax mediterranei
thermal denaturation is not reversible Haloferax mediterranei

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Haloferax mediterranei

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-aspartate + 2-oxoglutarate
-
 Haloferax mediterranei oxaloacetate + L-glutamate
-
 ?
L-aspartate + 2-oxoglutarate
-
 Haloferax mediterranei R-4 / ATCC 33500 oxaloacetate + L-glutamate
-
 ?

Synonyms

Synonyms Comment Organism
AspAT
-
 Haloferax mediterranei

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
 assay at Haloferax mediterranei
55
-
 0.7 M KCl Haloferax mediterranei
65
-
 1.6-3.4 M KCl Haloferax mediterranei

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
40 80 40°C: about 40% of activity maximum, 80°C: about 65% of activity maximum Haloferax mediterranei

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
additional information
-
 2-oxoglutarate protects against thermal inactivation Haloferax mediterranei
50
-
 under physiological or nearly physiological conditions, 3.5 M KCl, pH 7.8, stable for several h Haloferax mediterranei

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.8
-
 assay at Haloferax mediterranei