KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00025 | - |
pyridoxyl 5'-phosphate | pH 7.6, 25°C | Escherichia coli | |
0.071 | - |
2-oxoglutarate | pH 7.6, 25°C | Escherichia coli | |
0.37 | - |
oxaloacetate | pH 7.6, 25°C | Escherichia coli | |
1.43 | - |
L-tyrosine | pH 7.6, 25°C | Escherichia coli | |
2.17 | - |
L-phenylalanine | - |
Escherichia coli | |
4.4 | - |
L-aspartate | - |
Escherichia coli | |
5 | - |
L-tryptophan | pH 7.6, 25°C | Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
82000 | - |
PAGE | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-aspartate + 2-oxoglutarate | Escherichia coli | - |
oxaloacetate + L-glutamate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Escherichia coli |
Storage Stability | Organism |
---|---|
4°C, stable for several weeks | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-aspartate + 2-oxoglutarate | - |
Escherichia coli | oxaloacetate + L-glutamate | - |
? | |
L-aspartate + 2-oxoglutarate | - |
Escherichia coli | oxaloacetate + L-glutamate | - |
r | |
L-phenylalanine + 2-oxoglutarate | - |
Escherichia coli | 2-oxo-3-phenylpropionic acid + L-glutamate | i.e. phenylpyruvate | ? | |
L-tryptophan + 2-oxoglutarate | - |
Escherichia coli | 3-indole-2-oxopropionic acid + L-glutamate | - |
? | |
L-tyrosine + 2-oxoglutarate | - |
Escherichia coli | 3-(4-hydroxyphenyl)-2-oxopropionic acid + L-glutamate | i.e. 4-hydroxyphenylpyruvate | ? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 42000-45000, SDS-PAGE | Escherichia coli |
Synonyms | Comment | Organism |
---|---|---|
More | EC 2.6.1.57 may be converted to EC 2.6.1.1 by controlled proteolysis with subtilisin, the 2 enzymes are encoded by 2 different genes with high sequence homology | Escherichia coli |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
10 min, 48% loss of activity with tyrosine, 36% loss of activity with phenylalanine | Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
formation of aspartate | Escherichia coli |
8.5 | - |
substrates L-phenylalanine or L-tryptophan | Escherichia coli |
9 | - |
substrate L-tyrosine | Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | a pyridoxal 5'-phosphate protein | Escherichia coli |