Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.6.1.1 extracted from

  • Mavrides, C.; Orr, W.
    Multispecific aspartate and aromatic amino acid aminotransferases in Escherichia coli (1975), J. Biol. Chem., 250, 4128-4133.
    View publication on PubMed

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00025
-
pyridoxyl 5'-phosphate pH 7.6, 25°C Escherichia coli
0.071
-
2-oxoglutarate pH 7.6, 25°C Escherichia coli
0.37
-
oxaloacetate pH 7.6, 25°C Escherichia coli
1.43
-
L-tyrosine pH 7.6, 25°C Escherichia coli
2.17
-
L-phenylalanine
-
Escherichia coli
4.4
-
L-aspartate
-
Escherichia coli
5
-
L-tryptophan pH 7.6, 25°C Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
82000
-
PAGE Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-aspartate + 2-oxoglutarate Escherichia coli
-
oxaloacetate + L-glutamate
-
?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Escherichia coli

Storage Stability

Storage Stability Organism
4°C, stable for several weeks Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-aspartate + 2-oxoglutarate
-
Escherichia coli oxaloacetate + L-glutamate
-
?
L-aspartate + 2-oxoglutarate
-
Escherichia coli oxaloacetate + L-glutamate
-
r
L-phenylalanine + 2-oxoglutarate
-
Escherichia coli 2-oxo-3-phenylpropionic acid + L-glutamate i.e. phenylpyruvate ?
L-tryptophan + 2-oxoglutarate
-
Escherichia coli 3-indole-2-oxopropionic acid + L-glutamate
-
?
L-tyrosine + 2-oxoglutarate
-
Escherichia coli 3-(4-hydroxyphenyl)-2-oxopropionic acid + L-glutamate i.e. 4-hydroxyphenylpyruvate ?

Subunits

Subunits Comment Organism
dimer 2 * 42000-45000, SDS-PAGE Escherichia coli

Synonyms

Synonyms Comment Organism
More EC 2.6.1.57 may be converted to EC 2.6.1.1 by controlled proteolysis with subtilisin, the 2 enzymes are encoded by 2 different genes with high sequence homology Escherichia coli

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
55
-
10 min, 48% loss of activity with tyrosine, 36% loss of activity with phenylalanine Escherichia coli

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
formation of aspartate Escherichia coli
8.5
-
substrates L-phenylalanine or L-tryptophan Escherichia coli
9
-
substrate L-tyrosine Escherichia coli

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate a pyridoxal 5'-phosphate protein Escherichia coli