Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.5.1.9 extracted from

  • Illarionov, B.; Haase, I.; Fischer, M.; Bacher, A.; Schramek, N.
    Pre-steady-state kinetic analysis of riboflavin synthase using a pentacyclic reaction intermediate as substrate (2005), Biol. Chem., 386, 127-136.
    View publication on PubMed
Search for more literature for 2.5.1.9

Cloned(Commentary)

Cloned (Comment) Organism
expression of wild-type and mutant enzymes in strain BL21(DE3) Escherichia coli

Protein Variants

Protein Variants Comment Organism
F2A nearly inactive mutant, comparison of kinetics for wild-type and mutant enzymes Escherichia coli
H102Q highly reduced activity compared to the wild-type enzyme, comparison of kinetics for wild-type and mutant enzymes Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information steady-state and presteady-state kinetic analysis, single turnover conditions, transient stopped flow and quenched flow kinetics measurements with the pentacyclic reaction intermediate as substrate Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6,7-dimethyl-8-(1-D-ribityl)lumazine Escherichia coli
-
 riboflavin + 5-amino-6-ribitylamino-2,4-(1H,3H)-pyrimidinedione
-
 r

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Reaction

Reaction Comment Organism Reaction ID
2 6,7-dimethyl-8-(1-D-ribityl)lumazine = riboflavin + 4-(1-D-ribitylamino)-5-amino-2,6-dihydroxypyrimidine pentacyclic reaction intermediate Escherichia coli
a

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6,7-dimethyl-8-(1-D-ribityl)lumazine
-
 Escherichia coli riboflavin + 5-amino-6-ribitylamino-2,4-(1H,3H)-pyrimidinedione
-
 r
6,7-dimethyl-8-(1-D-ribityl)lumazine mechanistically complex dismutation Escherichia coli riboflavin + 5-amino-6-ribitylamino-2,4-(1H,3H)-pyrimidinedione
-
 r
additional information utilization of the pentacyclic reaction intermediate as substrate Escherichia coli ?
-
 ?

Subunits

Subunits Comment Organism
trimer
-
 Escherichia coli

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
 assay at Escherichia coli

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.001
-
 6,7-dimethyl-8-(1-D-ribityl)lumazine pH 6.9, 25°C, recombinant mutant H102Q, steady-state conditions Escherichia coli
0.267
-
 6,7-dimethyl-8-(1-D-ribityl)lumazine pH 6.9, 25°C, recombinant wild-type enzyme, steady-state conditions Escherichia coli

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.9
-
 assay at Escherichia coli