Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.5.1.9 extracted from

  • Eberhardt, S.; Korn, S.; Lottspeich, F.; Bacher, A.
    Biosynthesis of riboflavin: an unusual riboflavin synthase of Methanobacterium thermoautotrophicum (1997), J. Bacteriol., 179, 2938-2943.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
DNA and amino acid sequence determination and analysis, functional expression in riboflavin-deficient Escherichia coli mutant BSV23 Methanothermobacter thermautotrophicus

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ absolute requirement Methanothermobacter thermautotrophicus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6,7-dimethyl-8-ribityllumazine + 6,7-dimethyl-8-ribityllumazine Methanothermobacter thermautotrophicus
-
riboflavin + 4-(1'-D-ribitylamino)-5-amino-2,6-dihydroxypyrimidine
-
?
6,7-dimethyl-8-ribityllumazine + 6,7-dimethyl-8-ribityllumazine Methanothermobacter thermautotrophicus Marburg / DSM 2133
-
riboflavin + 4-(1'-D-ribitylamino)-5-amino-2,6-dihydroxypyrimidine
-
?

Organism

Organism UniProt Comment Textmining
Methanothermobacter thermautotrophicus Q59587 Marburg strain
-
Methanothermobacter thermautotrophicus Q59587 gene ribC
-
Methanothermobacter thermautotrophicus Marburg / DSM 2133 Q59587 Marburg strain
-

Purification (Commentary)

Purification (Comment) Organism
1500fold Methanothermobacter thermautotrophicus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.032
-
purified recombinant enzyme Methanothermobacter thermautotrophicus
0.032
-
65°C Methanothermobacter thermautotrophicus
0.045
-
purified enzyme Methanothermobacter thermautotrophicus
0.045
-
65°C Methanothermobacter thermautotrophicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6,7-dimethyl-8-ribityllumazine + 6,7-dimethyl-8-ribityllumazine
-
Methanothermobacter thermautotrophicus riboflavin + 4-(1'-D-ribitylamino)-5-amino-2,6-dihydroxypyrimidine
-
?
6,7-dimethyl-8-ribityllumazine + 6,7-dimethyl-8-ribityllumazine
-
Methanothermobacter thermautotrophicus Marburg / DSM 2133 riboflavin + 4-(1'-D-ribitylamino)-5-amino-2,6-dihydroxypyrimidine
-
?
additional information no activity with lumazine 5'-phosphate Methanothermobacter thermautotrophicus ?
-
?
additional information no activity with lumazine 5'-phosphate Methanothermobacter thermautotrophicus Marburg / DSM 2133 ?
-
?