Cloned (Comment) | Organism |
---|---|
DNA and amino acid sequence determination and analysis, functional expression in riboflavin-deficient Escherichia coli mutant BSV23 | Methanothermobacter thermautotrophicus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | absolute requirement | Methanothermobacter thermautotrophicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
6,7-dimethyl-8-ribityllumazine + 6,7-dimethyl-8-ribityllumazine | Methanothermobacter thermautotrophicus | - |
riboflavin + 4-(1'-D-ribitylamino)-5-amino-2,6-dihydroxypyrimidine | - |
? | |
6,7-dimethyl-8-ribityllumazine + 6,7-dimethyl-8-ribityllumazine | Methanothermobacter thermautotrophicus Marburg / DSM 2133 | - |
riboflavin + 4-(1'-D-ribitylamino)-5-amino-2,6-dihydroxypyrimidine | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Methanothermobacter thermautotrophicus | Q59587 | Marburg strain | - |
Methanothermobacter thermautotrophicus | Q59587 | gene ribC | - |
Methanothermobacter thermautotrophicus Marburg / DSM 2133 | Q59587 | Marburg strain | - |
Purification (Comment) | Organism |
---|---|
1500fold | Methanothermobacter thermautotrophicus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.032 | - |
purified recombinant enzyme | Methanothermobacter thermautotrophicus |
0.032 | - |
65°C | Methanothermobacter thermautotrophicus |
0.045 | - |
purified enzyme | Methanothermobacter thermautotrophicus |
0.045 | - |
65°C | Methanothermobacter thermautotrophicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
6,7-dimethyl-8-ribityllumazine + 6,7-dimethyl-8-ribityllumazine | - |
Methanothermobacter thermautotrophicus | riboflavin + 4-(1'-D-ribitylamino)-5-amino-2,6-dihydroxypyrimidine | - |
? | |
6,7-dimethyl-8-ribityllumazine + 6,7-dimethyl-8-ribityllumazine | - |
Methanothermobacter thermautotrophicus Marburg / DSM 2133 | riboflavin + 4-(1'-D-ribitylamino)-5-amino-2,6-dihydroxypyrimidine | - |
? | |
additional information | no activity with lumazine 5'-phosphate | Methanothermobacter thermautotrophicus | ? | - |
? | |
additional information | no activity with lumazine 5'-phosphate | Methanothermobacter thermautotrophicus Marburg / DSM 2133 | ? | - |
? |