KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | sigmoidal kinetics is observed with mixtures of artifactual beta 60 capsids and alpha subunit trimers. Km value for lumazine of the lumazine synthase/riboflavin synthase complex is about 10 times larger as compared to light riboflavin synthase consisting of alpha subunits, without lumazine synthase activity | Bacillus subtilis | |
0.13 | - |
6,7-dimethyl-8-(1'-D-ribityl)lumazine | pH 7.0, 37°C | Bacillus subtilis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus subtilis | - |
lumazine synthase/riboflavin synthase complex, the alpha subunit carries riboflavin synthase activity | - |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
2 6,7-dimethyl-8-(1-D-ribityl)lumazine = riboflavin + 4-(1-D-ribitylamino)-5-amino-2,6-dihydroxypyrimidine | formation of riboflavin by the lumazine synthase/riboflavin synthase complex follows a leaky channel model | Bacillus subtilis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
6,7-dimethyl-8-(1-D-ribityl)lumazine | - |
Bacillus subtilis | riboflavin + 5-amino-6-ribitylamino-2,4-(1H,3H)-pyrimidinedione | catalysed by the alpha-subunit, which forms a trimer surrounded by an icosahedral capsid of 60 beta subunits producing substrate 6,7-dimethyl-8-(1-D-ribityl)lumazine | ? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.019 | - |
6,7-dimethyl-8-ribityllumazine | pH 7.0, 37°C | Bacillus subtilis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
both lumazine synthase and riboflavin synthase activity | Bacillus subtilis |