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Literature summary for 2.5.1.9 extracted from

  • Plaut, G.W.E.; Harvey, R.A.
    The enzymatic synthesis of riboflavin (1971), Methods Enzymol., 18B, 515-538.
No PubMed abstract available
Search for more literature for 2.5.1.9

Inhibitors

Inhibitors Comment Organism Structure
2-Amino-4,6-dihydroxy-8-D-ribityl-7-pteridinone
-
 Saccharomyces cerevisiae
5,6,7,8-Tetrahydro-9-(1'-D-ribityl)isoalloxazine
-
 Saccharomyces cerevisiae
6,7-dihydroxy-8-ribityllumazine
-
 Saccharomyces cerevisiae
6,7-dimethyl-8-(1'-D-xylityl)lumazine
-
 Saccharomyces cerevisiae
6-Methyl-7-hydroxy-8-ribityllumazine
-
 Saccharomyces cerevisiae
p-chloromercuribenzenesulfonate reversible by cysteine or 2-mercaptoethanol Saccharomyces cerevisiae
riboflavin product inhibition, Saccharomyces cerevisiae Saccharomyces cerevisiae

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
70000 80000 analytical ultracentrifugation Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6,7-dimethyl-8-ribityllumazine + 6,7-dimethyl-8-ribityllumazine Lactiplantibacillus plantarum
-
 riboflavin + 4-(1'-D-ribitylamino)-5-amino-2,6-dihydroxypyrimidine
-
 ?
6,7-dimethyl-8-ribityllumazine + 6,7-dimethyl-8-ribityllumazine Bacillus subtilis
-
 riboflavin + 4-(1'-D-ribitylamino)-5-amino-2,6-dihydroxypyrimidine
-
 ?
6,7-dimethyl-8-ribityllumazine + 6,7-dimethyl-8-ribityllumazine Escherichia coli
-
 riboflavin + 4-(1'-D-ribitylamino)-5-amino-2,6-dihydroxypyrimidine
-
 ?
6,7-dimethyl-8-ribityllumazine + 6,7-dimethyl-8-ribityllumazine Saccharomyces cerevisiae
-
 riboflavin + 4-(1'-D-ribitylamino)-5-amino-2,6-dihydroxypyrimidine
-
 ?
6,7-dimethyl-8-ribityllumazine + 6,7-dimethyl-8-ribityllumazine Neurospora crassa
-
 riboflavin + 4-(1'-D-ribitylamino)-5-amino-2,6-dihydroxypyrimidine
-
 ?
6,7-dimethyl-8-ribityllumazine + 6,7-dimethyl-8-ribityllumazine Klebsiella aerogenes
-
 riboflavin + 4-(1'-D-ribitylamino)-5-amino-2,6-dihydroxypyrimidine
-
 ?
6,7-dimethyl-8-ribityllumazine + 6,7-dimethyl-8-ribityllumazine Pseudomonas sp.
-
 riboflavin + 4-(1'-D-ribitylamino)-5-amino-2,6-dihydroxypyrimidine
-
 ?
6,7-dimethyl-8-ribityllumazine + 6,7-dimethyl-8-ribityllumazine Eremothecium gossypii
-
 riboflavin + 4-(1'-D-ribitylamino)-5-amino-2,6-dihydroxypyrimidine
-
 ?

Organism

Organism UniProt Comment Textmining
Bacillus subtilis
-
-
-
Eremothecium gossypii
-
-
-
Escherichia coli
-
-
-
Klebsiella aerogenes
-
-
-
Lactiplantibacillus plantarum
-
-
-
Neurospora crassa
-
-
-
Pseudomonas sp.
-
-
-
Saccharomyces cerevisiae
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Saccharomyces cerevisiae

Reaction

Reaction Comment Organism Reaction ID
2 6,7-dimethyl-8-(1-D-ribityl)lumazine = riboflavin + 4-(1-D-ribitylamino)-5-amino-2,6-dihydroxypyrimidine reaction mechanism Lactiplantibacillus plantarum
a
2 6,7-dimethyl-8-(1-D-ribityl)lumazine = riboflavin + 4-(1-D-ribitylamino)-5-amino-2,6-dihydroxypyrimidine reaction mechanism Bacillus subtilis
a
2 6,7-dimethyl-8-(1-D-ribityl)lumazine = riboflavin + 4-(1-D-ribitylamino)-5-amino-2,6-dihydroxypyrimidine reaction mechanism Escherichia coli
a
2 6,7-dimethyl-8-(1-D-ribityl)lumazine = riboflavin + 4-(1-D-ribitylamino)-5-amino-2,6-dihydroxypyrimidine reaction mechanism Saccharomyces cerevisiae
a
2 6,7-dimethyl-8-(1-D-ribityl)lumazine = riboflavin + 4-(1-D-ribitylamino)-5-amino-2,6-dihydroxypyrimidine reaction mechanism Neurospora crassa
a
2 6,7-dimethyl-8-(1-D-ribityl)lumazine = riboflavin + 4-(1-D-ribitylamino)-5-amino-2,6-dihydroxypyrimidine reaction mechanism Klebsiella aerogenes
a
2 6,7-dimethyl-8-(1-D-ribityl)lumazine = riboflavin + 4-(1-D-ribitylamino)-5-amino-2,6-dihydroxypyrimidine reaction mechanism Pseudomonas sp.
a
2 6,7-dimethyl-8-(1-D-ribityl)lumazine = riboflavin + 4-(1-D-ribitylamino)-5-amino-2,6-dihydroxypyrimidine reaction mechanism Eremothecium gossypii
a

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Bacillus subtilis
additional information
-
-
 Saccharomyces cerevisiae
additional information
-
-
 Eremothecium gossypii

Storage Stability

Storage Stability Organism
-90°C or in liquid N2, stable for several months Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6,7-dimethyl-8-ribityllumazine + 6,7-dimethyl-8-ribityllumazine
-
 Lactiplantibacillus plantarum riboflavin + 4-(1'-D-ribitylamino)-5-amino-2,6-dihydroxypyrimidine
-
 ?
6,7-dimethyl-8-ribityllumazine + 6,7-dimethyl-8-ribityllumazine
-
 Bacillus subtilis riboflavin + 4-(1'-D-ribitylamino)-5-amino-2,6-dihydroxypyrimidine
-
 ?
6,7-dimethyl-8-ribityllumazine + 6,7-dimethyl-8-ribityllumazine
-
 Escherichia coli riboflavin + 4-(1'-D-ribitylamino)-5-amino-2,6-dihydroxypyrimidine
-
 ?
6,7-dimethyl-8-ribityllumazine + 6,7-dimethyl-8-ribityllumazine
-
 Saccharomyces cerevisiae riboflavin + 4-(1'-D-ribitylamino)-5-amino-2,6-dihydroxypyrimidine
-
 ?
6,7-dimethyl-8-ribityllumazine + 6,7-dimethyl-8-ribityllumazine
-
 Neurospora crassa riboflavin + 4-(1'-D-ribitylamino)-5-amino-2,6-dihydroxypyrimidine
-
 ?
6,7-dimethyl-8-ribityllumazine + 6,7-dimethyl-8-ribityllumazine
-
 Klebsiella aerogenes riboflavin + 4-(1'-D-ribitylamino)-5-amino-2,6-dihydroxypyrimidine
-
 ?
6,7-dimethyl-8-ribityllumazine + 6,7-dimethyl-8-ribityllumazine
-
 Pseudomonas sp. riboflavin + 4-(1'-D-ribitylamino)-5-amino-2,6-dihydroxypyrimidine
-
 ?
6,7-dimethyl-8-ribityllumazine + 6,7-dimethyl-8-ribityllumazine
-
 Eremothecium gossypii riboflavin + 4-(1'-D-ribitylamino)-5-amino-2,6-dihydroxypyrimidine
-
 ?
6,7-dimethyl-8-[1'-(5'-deoxy-D-ribityl)]lumazine + 6,7-dimethyl-8-[1'-(5'-deoxy-D-ribityl)]lumazine
-
 Saccharomyces cerevisiae 5'-deoxyriboflavin + ?
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Lactiplantibacillus plantarum
37
-
 assay at Bacillus subtilis
37
-
 assay at Escherichia coli
37
-
 assay at Saccharomyces cerevisiae
37
-
 assay at Neurospora crassa
37
-
 assay at Klebsiella aerogenes
37
-
 assay at Pseudomonas sp.
37
-
 assay at Eremothecium gossypii

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
 Bacillus subtilis
7
-
-
 Escherichia coli
7
-
-
 Saccharomyces cerevisiae

pH Range

pH Minimum pH Maximum Comment Organism
5.8 8.4 about 50% of activity maximum at pH 5.8 and 8.4 Saccharomyces cerevisiae

pH Stability

pH Stability pH Stability Maximum Comment Organism
4 8 0°C, stable for several h in presence of a suitable reducing agent Saccharomyces cerevisiae