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Literature summary for 2.5.1.67 extracted from

  • Rivera, S.B.; Swedlund, B.D.; King, G.J.; Bell, R.N.; Hussey, C.E., Jr.; Shattuck-Eidens, D.M.; Wrobel, W.M.; Peiser, G.D.; Poulter, C.D.
    Chrysanthemyl diphosphate synthase: isolation of the gene and characterization of the recombinant non-head-to-tail monoterpene synthase from Chrysanthemum cinerariaefolium (2001), Proc. Natl. Acad. Sci. USA, 98, 4373-4378.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.5.1.67

Cloned(Commentary)

Cloned (Comment) Organism
phage lambda cDNA library screening, DNA and amino acid sequence determination and analysis, functional overexpression of N-terminally His6-tagged enzyme in Escherichia coli strain XA90 Tanacetum cinerariifolium

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information steady-stae kinetics, the His6-tag of the recombinant enzyme does not influence the kinetics Tanacetum cinerariifolium

Localization

Localization Comment Organism GeneOntology No. Textmining
plastid
-
 Tanacetum cinerariifolium 9536
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required, 1-10 mM, can be substituted by Mn2+, best divalent cation Tanacetum cinerariifolium
Mn2+ activates, can substitute for Mg2+, optimal at 1 mM, less effective than Mg2+ Tanacetum cinerariifolium

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
41000
-
 1 * 45358, pre-protein, sequence calculation, 1 * 41000, recombinant enzyme, SDS-PAGE, 1 * 41200, recombinant enzyme, analytical sedimentation equilibrium ultracentrifugation Tanacetum cinerariifolium
41200
-
 1 * 45358, pre-protein, sequence calculation, 1 * 41000, recombinant enzyme, SDS-PAGE, 1 * 41200, recombinant enzyme, analytical sedimentation equilibrium ultracentrifugation Tanacetum cinerariifolium
45358
-
 1 * 45358, pre-protein, sequence calculation, 1 * 41000, recombinant enzyme, SDS-PAGE, 1 * 41200, recombinant enzyme, analytical sedimentation equilibrium ultracentrifugation Tanacetum cinerariifolium
66000
-
 recombinant enzyme, gel filtration Tanacetum cinerariifolium

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2 dimethylallyl diphosphate Tanacetum cinerariifolium
-
 diphosphate + chrysanthemyl diphosphate
-
 ?
additional information Tanacetum cinerariifolium irregular monoterpenes are common in Chrysanthemum cinerariaefolium and related members of the asteraceae family, CPP is an intermediate in the biosynthesis of the pyrethrin ester insecticides ?
-
 ?

Organism

Organism UniProt Comment Textmining
Tanacetum cinerariifolium P0C565 i.e. Tanacetum cinerariifolium, dalmatian daisy
-

Posttranslational Modification

Posttranslational Modification Comment Organism
proteolytic modification the pre-enzyme contains a putative plastidial targeting sequence Tanacetum cinerariifolium

Purification (Commentary)

Purification (Comment) Organism
native enzyme from immature flowers by ammonium sulfate fractionation, anion exchange and hydrophobic interaction chromatography, followed by another step of anion chromatography and hydroxylapatite chromatography to apparent homogeneity, recombinant N-terminally His6-tagged enzyme from Escherichia coli strain XA90 by ammonium sulfate fractionation and nickel affinity chromatography Tanacetum cinerariifolium

Source Tissue

Source Tissue Comment Organism Textmining
flower immature Tanacetum cinerariifolium
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.00267
-
 purified recombinant enzyme Tanacetum cinerariifolium

Storage Stability

Storage Stability Organism
-20°C, purified native enzyme in 30% glycerol for about 72 h with 20% decrease in activity Tanacetum cinerariifolium

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2 dimethylallyl diphosphate
-
 Tanacetum cinerariifolium diphosphate + chrysanthemyl diphosphate
-
 ?
2 dimethylallyl diphosphate
-
 Tanacetum cinerariifolium diphosphate + chrysanthemyl diphosphate product analysis, chrysanthemyl diphosphate is an irregular monoterpene with a non-head-to-tail or irregular c1'-2-3 linkage between isoprenoid units ?
additional information irregular monoterpenes are common in Chrysanthemum cinerariaefolium and related members of the asteraceae family, CPP is an intermediate in the biosynthesis of the pyrethrin ester insecticides Tanacetum cinerariifolium ?
-
 ?
additional information CPPase seems to be incapable of catalyzing 19-4 chain elongation despite its significant sequence homology to FPPase Tanacetum cinerariifolium ?
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 45358, pre-protein, sequence calculation, 1 * 41000, recombinant enzyme, SDS-PAGE, 1 * 41200, recombinant enzyme, analytical sedimentation equilibrium ultracentrifugation Tanacetum cinerariifolium

Synonyms

Synonyms Comment Organism
CPPase
-
 Tanacetum cinerariifolium

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Tanacetum cinerariifolium

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.008
-
 dimethylallyl diphosphate pH 7.5, 30°C, His-tagged or detagged recombinant enzyme Tanacetum cinerariifolium

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5 8
-
 Tanacetum cinerariifolium