Literature summary for 2.5.1.63 extracted from

Dong, C.; Huang, F.; Deng, H.; Schaffrath, C.; Spencer, J.B.; O'Hagan, D.; Naismith, J.H.
Crystal structure and mechanism of a bacterial fluorinating enzyme (2004), Nature, 427, 561-565.

Search for more literature for 2.5.1.63

Cloned(Commentary)

Cloned (Comment)	Organism
gene flA, DNA sequence determination and analysis, overexpression of His-tagged enzyme in Escherichia coli strain BL21(DE3) and in strain B834(DE3), the latter results in the selenomethionine labeled enzyme	Streptomyces cattleya

Crystallization (Commentary)

Crystallization (Comment)	Organism
purified recombinant wild-type and selenomethionine labelled enzyme with bound S-adenosyl-L-methionine, X-ray diffraction structure determination and analysis at 1.9 A resolution, structure modelling of monomers a and b	Streptomyces cattleya

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
34402	-	6 * 34402, (alpha,beta)3, recombinant enzyme, ESI-MS mass spectrometry	Streptomyces cattleya

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
S-adenosyl-L-methionine + fluoride	Streptomyces cattleya	-	5'-deoxy-5'-fluoroadenosine + L-methionine	-	?

Organism

Organism	UniProt	Comment	Textmining
Streptomyces cattleya	-	gene flA	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant His-tagged enzyme from Escherichia coli by nickel affinity chromatography and gel filtration	Streptomyces cattleya

Reaction

Reaction	Comment	Organism	Reaction ID
S-adenosyl-L-methionine + fluoride = 5'-deoxy-5'-fluoroadenosine + L-methionine	substrate binding structure involving F156, S158, Y177, and T80, nucleophilic substitution reaction mechanism	Streptomyces cattleya	a

Source Tissue

Source Tissue	Comment	Organism	Textmining
culture condition:fluoride-grown cell	-	Streptomyces cattleya	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
S-adenosyl-L-methionine + fluoride	-	Streptomyces cattleya	5'-deoxy-5'-fluoroadenosine + L-methionine	-	?
S-adenosyl-L-methionine + fluoride	highly specific for S-adenosyl-L-methionine, which is tightly bound to the enzyme at the interface between C-terminal domain of one monomer and the N-terminal domain of the neighbouring monomer	Streptomyces cattleya	5'-deoxy-5'-fluoroadenosine + L-methionine	-	?

Subunits

Subunits	Comment	Organism
hexamer	6 * 34402, (alpha,beta)3, recombinant enzyme, ESI-MS mass spectrometry	Streptomyces cattleya
More	the N-terminal domain has a central seven-stranded beta-sheet, which combines parallel and antiparallel strands sandwiched between alpha-helices, the C-terminal domain is composed of a five- and a four-stranded antiparallel beta-sheet	Streptomyces cattleya

Synonyms

Synonyms	Comment	Organism
5'-deoxy-5'-fluoroadenosine synthase	-	Streptomyces cattleya
5'-FDAS	-	Streptomyces cattleya
fluorination enzyme	-	Streptomyces cattleya

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
37	-	assay at	Streptomyces cattleya

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.0012	-	fluoride	-	Streptomyces cattleya
0.074	-	S-adenosyl-L-methionine	-	Streptomyces cattleya
2	-	fluoride	-	Streptomyces cattleya

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.9	-	assay at	Streptomyces cattleya

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)