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Literature summary for 2.5.1.62 extracted from
- Competitive inhibitions of the chlorophyll synthase of Synechocystis sp. strain PCC 6803 by bacteriochlorophyllide a and the bacteriochlorophyll synthase of Rhodobacter sphaeroides by chlorophyllide a (2010), J. Bacteriol., 192, 198-207.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| PCR-amplification, in T-vector, expression plasmid pRSET-A in Escherichia coli BL21(DE3)/pLysS | Synechocystis sp. PCC 6803 |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| bacteriochlorophyllide a | competitive inhibitor, effect is reversed by an increased level of chlroophyllide a, no cell growth of 50 microM | Synechocystis sp. PCC 6803 |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.1 | - |
geranylgeranyl diphosphate | lysate mixed into reaction buffer (120 mM potassium acetate, 10 mM magnesium acetate, 50 mM HEPES-KOH, pH 7.6, 14 mM 2-mercaptoethanol, 10% glycerol, and 0.5 mM ATP), 30°C | Synechocystis sp. PCC 6803 | |
| 0.32 | - |
phytyl diphosphate | lysate mixed into reaction buffer (120 mM potassium acetate, 10 mM magnesium acetate, 50 mM HEPES-KOH, pH 7.6, 14 mM 2-mercaptoethanol, 10% glycerol, and 0.5 mM ATP), 30°C | Synechocystis sp. PCC 6803 | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| chlorophyllide a + geranylgeranyl diphosphate | Synechocystis sp. PCC 6803 | - |
geranylgeranylchlorophyllide a + diphosphate | - |
? | |
| chlorophyllide a + phytyl diphosphate | Synechocystis sp. PCC 6803 | - |
chlorophyll a + diphosphate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Synechocystis sp. PCC 6803 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| cell harvesting, washing with 10 mM Tris-HCl, pH 8.0, freeze-thaw-cycles, incubated with DNase and 5 mM MgCl2 at room temperature, lysate used for assays | Synechocystis sp. PCC 6803 |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| chlorophyllide a + geranylgeranyl diphosphate | - |
Synechocystis sp. PCC 6803 | geranylgeranylchlorophyllide a + diphosphate | - |
? | |
| chlorophyllide a + phytyl diphosphate | - |
Synechocystis sp. PCC 6803 | chlorophyll a + diphosphate | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| ChlG | - |
Synechocystis sp. PCC 6803 |
| chlorophyll synthase | - |
Synechocystis sp. PCC 6803 |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.3 | - |
bacteriochlorophyllide a | in the presence of sufficient geranylgeranyl diphosphate as substrate, lysate mixed into reaction buffer (120 mM potassium acetate, 10 mM magnesium acetate, 50 mM HEPES-KOH, pH 7.6, 14 mM 2-mercaptoethanol, 10% glycerol, and 0.5 mM ATP), 30°C | Synechocystis sp. PCC 6803 | |
| 1.14 | - |
bacteriochlorophyllide a | in the presence of sufficient phytyl diphosphate as substrate, lysate mixed into reaction buffer (120 mM potassium acetate, 10 mM magnesium acetate, 50 mM HEPES-KOH, pH 7.6, 14 mM 2-mercaptoethanol, 10% glycerol, and 0.5 mM ATP), 30°C | Synechocystis sp. PCC 6803 | |
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