Activating Compound | Comment | Organism | Structure |
---|---|---|---|
Nonidet P-40 | in assay requirement | Saccharomyces cerevisiae |
Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Saccharomyces cerevisiae |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Zn2+ | at concentrations above 0.01 mM | Saccharomyces cerevisiae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0011 | - |
Ypt1p | pH 7.3, 30°C | Saccharomyces cerevisiae | |
0.0016 | - |
geranylgeranyl diphosphate | pH 7.3, 30°C | Saccharomyces cerevisiae |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | requirement, optimal concentration at 8 mM | Saccharomyces cerevisiae | |
Zn2+ | requirement, optimal concentration at 0.008 mM, zinc content: 0.7-0.12 mol/mol enzyme | Saccharomyces cerevisiae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | - |
- |
- |
Saccharomyces cerevisiae JRY1550 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant enzyme | Saccharomyces cerevisiae |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
geranylgeranyl diphosphate + protein-cysteine = S-geranylgeranyl-protein + diphosphate | enzyme is unique in the protein prenyltransferase family, consisting protein farnesyltransferase, EC 2.5.1.58, protein geranylgeranyltransferase type I, EC 2.5.1.59 and Rab geranylgeranyltransferase | Saccharomyces cerevisiae |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.0082 | - |
- |
Saccharomyces cerevisiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
geranylgeranyl diphosphate + protein-cysteine | enzyme attaches geranylgeranyl groups to two C-terminal cysteines in Ras-related GTPases of a single family, the Rab family, Ypt/Sec4 in lower eukaryotes, that terminate in XXCC, XCXC and CCXX motifs, reaction only if Rab is bound to a carrier protein termed Rab escort protein, REP | Saccharomyces cerevisiae | S-geranylgeranyl-protein + diphosphate | - |
? | |
geranylgeranyl diphosphate + protein-cysteine | enzyme attaches geranylgeranyl groups to two C-terminal cysteines in Ras-related GTPases of a single family, the Rab family, Ypt/Sec4 in lower eukaryotes, that terminate in XXCC, XCXC and CCXX motifs, reaction only if Rab is bound to a carrier protein termed Rab escort protein, REP | Saccharomyces cerevisiae JRY1550 | S-geranylgeranyl-protein + diphosphate | - |
? | |
geranylgeranyl diphosphate + Ypt1p | yeast enzyme catalyses the prenylation of Ypt1p in the presence of an escort protein, Msi4p | Saccharomyces cerevisiae | ? | - |
? | |
geranylgeranyl diphosphate + Ypt1p | yeast enzyme catalyses the prenylation of Ypt1p in the presence of an escort protein, Msi4p | Saccharomyces cerevisiae JRY1550 | ? | - |
? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | 7.5 | - |
Saccharomyces cerevisiae |