Protein Variants | Comment | Organism |
---|---|---|
C21N | complete loss of activity | Acidithiobacillus ferrooxidans |
D243A | mutation of the absolutely conserved 243AspGlyPro245 motif, complete loss of activity | Acidithiobacillus ferrooxidans |
D243Q | mutation of the absolutely conserved 243AspGlyPro245 motif, active enzyme with altered metal-dependency | Acidithiobacillus ferrooxidans |
P245A | mutation of the absolutely conserved 243AspGlyPro245 motif, active enzyme with altered metal-dependency | Acidithiobacillus ferrooxidans |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0052 | - |
phosphoenolpyruvate | mutant P245A, pH 7.2, 37°C | Acidithiobacillus ferrooxidans | |
0.012 | - |
phosphoenolpyruvate | wild-type, pH 7.2, 37°C | Acidithiobacillus ferrooxidans | |
0.013 | - |
phosphoenolpyruvate | mutant D243E, pH 7.2, 37°C | Acidithiobacillus ferrooxidans | |
0.021 | - |
D-arabinose 5-phosphate | wild-type, pH 7.2, 37°C | Acidithiobacillus ferrooxidans | |
0.024 | - |
D-arabinose 5-phosphate | mutant P245A, pH 7.2, 37°C | Acidithiobacillus ferrooxidans | |
2.63 | - |
D-arabinose 5-phosphate | mutant D243E, pH 7.2, 37°C | Acidithiobacillus ferrooxidans |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Cd2+ | presence of Cd2+ restores activity to metal-free enzyme, Cd2+ significantly enhances the stability of the enzyme and raises the Tm by 14°C | Acidithiobacillus ferrooxidans | |
Co2+ | presence of Co2+ restores activity to metal-free enzyme | Acidithiobacillus ferrooxidans | |
Mn2+ | presence of Mn2+ restores activity to metal-free enzyme | Acidithiobacillus ferrooxidans | |
additional information | divalent metal ion required, with Mn2+, Co2+ and Cd2+ in decreasing order being able to restore activity to metal-free enzyme | Acidithiobacillus ferrooxidans |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Acidithiobacillus ferrooxidans | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | D-glucose 6-phosphate and D-erythrose 4-phosphate are no substrates | Acidithiobacillus ferrooxidans | ? | - |
? | |
phosphoenolpyruvate + 2-deoxy-D-ribose 5-phosphate | 2-deoxy-D-ribose 5-phosphate is a poor substrate | Acidithiobacillus ferrooxidans | ? + phosphate | - |
? | |
phosphoenolpyruvate + D-arabinose 5-phosphate | - |
Acidithiobacillus ferrooxidans | 2-dehydro-3-deoxy-D-octonate 8-phosphate + phosphate | - |
? |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
68 | - |
wild-type, melting temperature | Acidithiobacillus ferrooxidans |
68 | - |
mutant C21N, presence of Cd2+, melting temperature | Acidithiobacillus ferrooxidans |
68 | - |
mutant D243E, melting temperature | Acidithiobacillus ferrooxidans |
69 | - |
mutant C21N, melting temperature | Acidithiobacillus ferrooxidans |
71 | - |
mutant P245A, melting temperature | Acidithiobacillus ferrooxidans |
78 | - |
mutant D243E, presence of Cd2+, melting temperature | Acidithiobacillus ferrooxidans |
79 | - |
mutant P245A, presence of Cd2+, melting temperature | Acidithiobacillus ferrooxidans |
82 | - |
wild-type, presence of Cd2+, melting temperature | Acidithiobacillus ferrooxidans |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
4.4 | - |
D-arabinose 5-phosphate | mutant P245A, pH 7.2, 37°C | Acidithiobacillus ferrooxidans | |
4.8 | - |
D-arabinose 5-phosphate | wild-type, pH 7.2, 37°C | Acidithiobacillus ferrooxidans | |
4.8 | - |
D-arabinose 5-phosphate | mutant D243E, pH 7.2, 37°C | Acidithiobacillus ferrooxidans |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.8 | - |
D-arabinose 5-phosphate | mutant D243E, pH 7.2, 37°C | Acidithiobacillus ferrooxidans | |
180 | - |
D-arabinose 5-phosphate | mutant P245A, pH 7.2, 37°C | Acidithiobacillus ferrooxidans | |
230 | - |
D-arabinose 5-phosphate | wild-type, pH 7.2, 37°C | Acidithiobacillus ferrooxidans | |
370 | - |
phosphoenolpyruvate | mutant D243E, pH 7.2, 37°C | Acidithiobacillus ferrooxidans | |
400 | - |
phosphoenolpyruvate | wild-type, pH 7.2, 37°C | Acidithiobacillus ferrooxidans | |
850 | - |
phosphoenolpyruvate | mutant P245A, pH 7.2, 37°C | Acidithiobacillus ferrooxidans |