Literature summary for 2.5.1.55 extracted from

Shulami, S.; Furdui, C.; Adir, N.; Shoham, Y.; Anderson, K.S.; Baasov, T.
A reciprocal single mutation affects the metal requirement of 3-deoxy-D-manno-2-octulosonate-8-phosphate (KDO8P) synthases from Aquifex pyrophilus and Escherichia coli (2004), J. Biol. Chem., 279, 45110-45120.

Search for more literature for 2.5.1.55

Activating Compound

Activating Compound	Comment	Organism	Structure
EDTA	-	Escherichia coli

Protein Variants

Protein Variants	Comment	Organism
C11N	enzyme retains 10% of the wild-type activity in absence of metal ions. Addition of divalent metal ions does not affect the catalytic activity of the mutant enzyme and the catalytic efficiency, i.e. the ratio of turnover number to Km-value, is reduced only 12fold, the mutant enzyme has become metal-independent	Aquifex pyrophilus
N26C	activity of the wild-type enzyme is independent of metal ions, the activity of mutant enzyme is decreased by EDTA and increased by Mn2+ and Cd2+	Escherichia coli

Inhibitors

Inhibitors	Comment	Organism	Structure
1,10-phenanthroline	inhibits wild-type enzyme, no inhibition of mutant enzyme C11N	Aquifex pyrophilus
EDTA	inhibits wild-type enzyme, no inhibition of mutant enzyme C11N	Aquifex pyrophilus

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.0058	-	phosphoenolpyruvate	pH 7.5, 37°C, Cd2+ mutant enzyme N26C	Escherichia coli
0.006	-	phosphoenolpyruvate	pH 7.5, 37°C, wild-type enzyme	Escherichia coli
0.017	-	phosphoenolpyruvate	pH 7.5, 60°C, mutant enzyme C11N, EDTA-treated	Aquifex pyrophilus
0.019	-	phosphoenolpyruvate	pH 7.5, 37°C, Mn2+ mutant enzyme N26C	Escherichia coli
0.02	-	D-arabinose 5-phosphate	pH 7.5, 37°C, wild-type enzyme	Escherichia coli
0.026	-	phosphoenolpyruvate	pH 7.5, 60°C, wild-type Mn2+-enzyme	Aquifex pyrophilus
0.032	-	phosphoenolpyruvate	pH 7.5, 37°C, mutant enzyme N26C, EDTA-treated	Escherichia coli
0.067	-	D-arabinose 5-phosphate	pH 7.5, 60°C, wild-type Mn2+-enzyme	Aquifex pyrophilus
0.07	-	D-arabinose 5-phosphate	pH 7.5, 37°C, Mn2+ mutant enzyme N26C	Escherichia coli
0.075	-	D-arabinose 5-phosphate	pH 7.5, 37°C, mutant enzyme N26C, EDTA-treated	Escherichia coli
0.11	-	D-arabinose 5-phosphate	pH 7.5, 37°C, Cd2+ mutant enzyme N26C	Escherichia coli
0.14	-	D-arabinose 5-phosphate	pH 7.5, 60°C, mutant enzyme C11N, EDTA-treated	Aquifex pyrophilus

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Cd2+	increase in steady-state rate of wild-type enzyme, Km: 0.0006 mM	Aquifex pyrophilus
Iron	wild-type enzyme contains 0.19 mol of iron per mol of enzyme, mutant enzyme c11N contains 0.22 mol of iron per mol of enzyme	Aquifex pyrophilus
Magnesium	wild-type enzyme contains 0.05 mol of magnesium per mol of enzyme, mutant enzyme N26C contains 0.4 mol of magnesium per mol of enzyme	Escherichia coli
Mn2+	200fold increase in steady-state rate of wild-type enzyme, Km: 0.01 mM. Addition of Mn2+ up to 2 mM does not stimulate the reaction rate of C11N mutant	Aquifex pyrophilus
Zinc	wild-type enzyme contains 0.05 mol of zinc per mol of enzyme, mutant enzyme N26C contains 0.2 mol of zinc per mol of enzyme	Escherichia coli
Zinc	wild-type enzyme contains 0.26 mol per mol of enzyme, mutant enzyme c11N contains 0.02 mol of zinc per mol of enzyme.	Aquifex pyrophilus

Organism

Organism	UniProt	Comment	Textmining
Aquifex pyrophilus	Q8GLK7	-	-
Escherichia coli	P0A715	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
phosphoenolpyruvate + D-arabinose 5-phosphate	-	Escherichia coli	2-dehydro-3-deoxy-D-octonate 8-phosphate + phosphate	-	?
phosphoenolpyruvate + D-arabinose 5-phosphate	-	Aquifex pyrophilus	2-dehydro-3-deoxy-D-octonate 8-phosphate + phosphate	-	?

Synonyms

Synonyms	Comment	Organism
3-deoxy-D-manno-2-octulosonate-8-phosphate synthase	-	Escherichia coli
3-deoxy-D-manno-2-octulosonate-8-phosphate synthase	-	Aquifex pyrophilus
Kdo8P synthase	-	Escherichia coli
Kdo8P synthase	-	Aquifex pyrophilus

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.36	-	phosphoenolpyruvate	pH 7.5, 37°C, mutant enzyme N26C, EDTA-treated	Escherichia coli
0.36	-	D-arabinose 5-phosphate	pH 7.5, 37°C, mutant enzyme N26C, EDTA-treated	Escherichia coli
0.42	-	phosphoenolpyruvate	pH 7.5, 60°C, mutant enzyme C11N, EDTA-treated	Aquifex pyrophilus
0.42	-	D-arabinose 5-phosphate	pH 7.5, 60°C, mutant enzyme C11N, EDTA-treated	Aquifex pyrophilus
1.9	-	phosphoenolpyruvate	pH 7.5, 37°C, Cd2+ mutant enzyme N26C	Escherichia coli
1.9	-	D-arabinose 5-phosphate	pH 7.5, 37°C, Cd2+ mutant enzyme N26C	Escherichia coli
1.9	-	phosphoenolpyruvate	pH 7.5, 37°C, Mn2+ mutant enzyme N26C	Escherichia coli
1.9	-	D-arabinose 5-phosphate	pH 7.5, 37°C, Mn2+ mutant enzyme N26C	Escherichia coli
6.1	-	phosphoenolpyruvate	pH 7.5, 37°C, wild-type enzyme	Escherichia coli
6.1	-	D-arabinose 5-phosphate	pH 7.5, 37°C, wild-type enzyme	Escherichia coli
9	-	phosphoenolpyruvate	pH 7.5, 60°C, wild-type Mn2+-enzyme	Aquifex pyrophilus
9	-	D-arabinose 5-phosphate	pH 7.5, 60°C, wild-type Mn2+-enzyme	Aquifex pyrophilus

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Release 2023.1 (January 2023)