Cloned (Comment) | Organism |
---|---|
enzyme from wild-type strain AG701 and temperature-sensitive strain AG701i50 expressed in Escherichia coli | Salmonella enterica subsp. enterica serovar Typhimurium |
Protein Variants | Comment | Organism |
---|---|---|
P145S | natural mutation in the temperature-sensitive strain AG701i50 leads to an increase in Km-value for both substrates, D-arabinose 5-phosphate and phosphoenolpyruvate, this mutant enzyme also has an altered oligomeric state. Reduced activity, about 35% of the wild-type, between 15 and 30°C. Above 30°C the activity of the mutant enzyme decreases dramatically | Salmonella enterica subsp. enterica serovar Typhimurium |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0028 | - |
phosphoenolpyruvate | pH 7.4, 25°C, wild-type enzyme | Salmonella enterica subsp. enterica serovar Typhimurium | |
0.0042 | - |
phosphoenolpyruvate | pH 7.4, 37°C, wild-type enzyme | Salmonella enterica subsp. enterica serovar Typhimurium | |
0.0044 | - |
D-arabinose 5-phosphate | pH 7.4, 25°C, wild-type enzyme | Salmonella enterica subsp. enterica serovar Typhimurium | |
0.0093 | - |
D-arabinose 5-phosphate | pH 7.4, 37°C, wild-type enzyme | Salmonella enterica subsp. enterica serovar Typhimurium | |
0.014 | - |
D-arabinose 5-phosphate | pH 7.4, 25°C, mutant enzyme P145S | Salmonella enterica subsp. enterica serovar Typhimurium | |
0.26 | - |
phosphoenolpyruvate | pH 7.4, 25°C, mutant enzyme P145S | Salmonella enterica subsp. enterica serovar Typhimurium | |
0.28 | - |
D-arabinose 5-phosphate | pH 7.4, 37°C, mutant enzyme P145S | Salmonella enterica subsp. enterica serovar Typhimurium | |
1.65 | - |
phosphoenolpyruvate | pH 7.4, 37°C, mutant enzyme P145S | Salmonella enterica subsp. enterica serovar Typhimurium |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
additional information | - |
two poorly resolved peaks are detected by gel filtration of the mutant enzyme P145S: 91000 Da and 53000 Da | Salmonella enterica subsp. enterica serovar Typhimurium |
97000 | - |
wild-type enzyme, gel filtration | Salmonella enterica subsp. enterica serovar Typhimurium |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Salmonella enterica subsp. enterica serovar Typhimurium | - |
wild-type strain AG701 and temperature-sensitive strain AG701i50 | - |
Purification (Comment) | Organism |
---|---|
recombinant enzyme from wild-type strain AG701 and temperature-sensitive strain AG701i50 | Salmonella enterica subsp. enterica serovar Typhimurium |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
phosphoenolpyruvate + D-arabinose 5-phosphate | - |
Salmonella enterica subsp. enterica serovar Typhimurium | 2-dehydro-3-deoxy-D-octonate 8-phosphate + phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
trimer | - |
Salmonella enterica subsp. enterica serovar Typhimurium |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
1 h, wild-type enzyme retains full activity, 50% loss of activity of the mutant enzyme P145S | Salmonella enterica subsp. enterica serovar Typhimurium |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.72 | - |
phosphoenolpyruvate | pH 7.4, 25°C, mutant enzyme P145S | Salmonella enterica subsp. enterica serovar Typhimurium | |
0.74 | - |
D-arabinose 5-phosphate | pH 7.4, 25°C, mutant enzyme P145S | Salmonella enterica subsp. enterica serovar Typhimurium | |
1.63 | - |
D-arabinose 5-phosphate | pH 7.4, 37°C, mutant enzyme P145S | Salmonella enterica subsp. enterica serovar Typhimurium | |
1.75 | - |
D-arabinose 5-phosphate | pH 7.4, 25°C, wild-type enzyme | Salmonella enterica subsp. enterica serovar Typhimurium | |
2.02 | - |
phosphoenolpyruvate | pH 7.4, 37°C, mutant enzyme P145S | Salmonella enterica subsp. enterica serovar Typhimurium | |
2.08 | - |
phosphoenolpyruvate | pH 7.4, 25°C, wild-type enzyme | Salmonella enterica subsp. enterica serovar Typhimurium | |
5.6 | - |
D-arabinose 5-phosphate | pH 7.4, 37°C, wild-type enzyme | Salmonella enterica subsp. enterica serovar Typhimurium | |
6.7 | - |
phosphoenolpyruvate | pH 7.4, 37°C, wild-type enzyme | Salmonella enterica subsp. enterica serovar Typhimurium |