Crystallization (Comment) | Organism |
---|---|
hanging-drop vapor diffusion, ctystal structure of wild-type enzyme and mutant enzyme I181D | Pyrococcus furiosus |
Protein Variants | Comment | Organism |
---|---|---|
I181D | mutant enzyme is catalytically more active than the wild type enzyme from 20 to 80°C, the mutation disrupts the tetrameric structure of the enzyme, the melting temperatures of the wild-type protein are significantly higher than the melting temperatures of mutant enzyme I181D at pH values greater than 6.5 | Pyrococcus furiosus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.066 | - |
phosphoenolpyruvate | pH 7.5, 60°C, mutant enzyme I181D | Pyrococcus furiosus | |
0.071 | - |
D-erythrose 4-phosphate | pH 7.5, 60°C, mutant enzyme I181D | Pyrococcus furiosus | |
0.092 | - |
D-erythrose 4-phosphate | pH 7.5, 60°C, wild-type enzyme | Pyrococcus furiosus | |
0.112 | - |
phosphoenolpyruvate | pH 7.5, 60°C, wild-type enzyme | Pyrococcus furiosus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
109000 | - |
sedimentation equilibrium experiments, tetrameric enzyme | Pyrococcus furiosus |
111000 | - |
gel filtration, tetrameric enzyme | Pyrococcus furiosus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pyrococcus furiosus | Q8U0A9 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
phosphoenolpyruvate + D-erythrose 4-phosphate + H2O | - |
Pyrococcus furiosus | 3-deoxy-D-arabino-hept-2-ulosonate 7-phosphate + phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | although the enzyme crystallizes as a tetramer, equilibrium exists between tetrameric and dimeric forms with a dissociation constant of 0.022 mM | Pyrococcus furiosus |
tetramer | at pH 7.5 and 20°C, the wild-type enzyme is present as a tetramer in solution. Although the enzyme crystallizes as a tetramer, equilibrium exists between tetrameric and dimeric forms with a dissociation constant of 0.022 mM | Pyrococcus furiosus |
Synonyms | Comment | Organism |
---|---|---|
3-deoxy-D-arabino-heptulosonate-7-phosphate synthase | - |
Pyrococcus furiosus |
DAH7PS | - |
Pyrococcus furiosus |
PF1690 | locus name | Pyrococcus furiosus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
assay at | Pyrococcus furiosus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
98 | - |
melting temperature of the wild-type enzyme by differential scanning calorimetry at a protein monomer concentration of 0.034 mM and a pH of 7.5 | Pyrococcus furiosus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
5.5 | - |
phosphoenolpyruvate | pH 7.5, 60°C, wild-type enzyme | Pyrococcus furiosus | |
5.5 | - |
D-erythrose 4-phosphate | pH 7.5, 60°C, wild-type enzyme | Pyrococcus furiosus | |
14.9 | - |
phosphoenolpyruvate | pH 7.5, 60°C, mutant enzyme I181D | Pyrococcus furiosus | |
14.9 | - |
D-erythrose 4-phosphate | pH 7.5, 60°C, mutant enzyme I181D | Pyrococcus furiosus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Pyrococcus furiosus |
7.5 | - |
mutant enzyme I181D | Pyrococcus furiosus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 9 | at pH 9.0 the activity is about 60% of the activity at pH 6.0, wid-type enzyme | Pyrococcus furiosus |
6 | 9 | pH 6.0: about 55% of maximal activity, pH 9.0: about 35% of maximal activity, mutant enzyme I181D | Pyrococcus furiosus |
General Information | Comment | Organism |
---|---|---|
physiological function | the enzyme is involved in shikimate biosynthesis pathway | Pyrococcus furiosus |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.21 | - |
D-erythrose 4-phosphate | pH 7.5, 60°C, mutant enzyme I181D | Pyrococcus furiosus | |
0.22 | - |
phosphoenolpyruvate | pH 7.5, 60°C, mutant enzyme I181D | Pyrococcus furiosus | |
49 | - |
phosphoenolpyruvate | pH 7.5, 60°C, wild-type enzyme | Pyrococcus furiosus | |
60 | - |
D-erythrose 4-phosphate | pH 7.5, 60°C, wild-type enzyme | Pyrococcus furiosus |