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Literature summary for 2.5.1.54 extracted from

  • Ma, N.; Wei, L.; Fan, Y.; Hua, Q.
    Heterologous expression and characterization of soluble recombinant 3-deoxy-D-arabino-heptulosonate-7-phosphate synthase from Actinosynnema pretiosum ssp. auranticum ATCC31565 through co-expression with Chaperones in Escherichia coli (2012), Protein Expr. Purif., 82, 263-269.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in soluble form by co-expression with chaperonins GroEL/GroES in Escherichia coli Actinosynnema pretiosum subsp. auranticum

Inhibitors

Inhibitors Comment Organism Structure
EDTA 2 mM, complete inactivation within 10 min Actinosynnema pretiosum subsp. auranticum
additional information substrate D-erythrose 4-phosphate is not inhibitory Actinosynnema pretiosum subsp. auranticum
phosphoenolpyruvate pronounced substrate inhibition above 1 mM Actinosynnema pretiosum subsp. auranticum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.09
-
phosphoenolpyruvate pH 7.5, 30°C Actinosynnema pretiosum subsp. auranticum
0.43
-
D-erythrose 4-phosphate pH 7.5, 30°C Actinosynnema pretiosum subsp. auranticum

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ 0.1 mM, up to 500% of initial activity Actinosynnema pretiosum subsp. auranticum
Co2+ 0.1 mM, up to 535% of initial activity Actinosynnema pretiosum subsp. auranticum
Mn2+ 0.1 mM, up to 550% of initial activity Actinosynnema pretiosum subsp. auranticum
Ni2+ 0.1 mM, up to 495% of initial activity Actinosynnema pretiosum subsp. auranticum

Organism

Organism UniProt Comment Textmining
Actinosynnema pretiosum subsp. auranticum O68903
-
-
Actinosynnema pretiosum subsp. auranticum ATCC 31565 O68903
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.0127
-
pH 7.5, 30°C Actinosynnema pretiosum subsp. auranticum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
phosphoenolpyruvate + D-erythrose 4-phosphate + H2O
-
Actinosynnema pretiosum subsp. auranticum 3-deoxy-D-arabino-hept-2-ulosonate 7-phosphate + phosphate
-
?
phosphoenolpyruvate + D-erythrose 4-phosphate + H2O
-
Actinosynnema pretiosum subsp. auranticum ATCC 31565 3-deoxy-D-arabino-hept-2-ulosonate 7-phosphate + phosphate
-
?

Synonyms

Synonyms Comment Organism
aroF
-
Actinosynnema pretiosum subsp. auranticum

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.03
-
D-erythrose 4-phosphate pH 7.5, 30°C Actinosynnema pretiosum subsp. auranticum
0.032
-
phosphoenolpyruvate pH 7.5, 30°C Actinosynnema pretiosum subsp. auranticum

pI Value

Organism Comment pI Value Maximum pI Value
Actinosynnema pretiosum subsp. auranticum predicted
-
5.5

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.069
-
D-erythrose 4-phosphate pH 7.5, 30°C Actinosynnema pretiosum subsp. auranticum
0.35
-
phosphoenolpyruvate pH 7.5, 30°C Actinosynnema pretiosum subsp. auranticum