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Literature summary for 2.5.1.54 extracted from

  • Zhou, L.; Wu, J.; Janakiraman, V.; Shumilin, I.A.; Bauerle, R.; Kretsinger, R.H.; Woodard, R.W.
    Structure and characterization of the 3-deoxy-D-arabino-heptulosonate 7-phosphate synthase from Aeropyrum pernix (2012), Bioorg. Chem., 40, 79-86.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Aeropyrum pernix

Crystallization (Commentary)

Crystallization (Comment) Organism
in complex with a manganese ion and phosphoenolpyruvate. Crystals contain a tetramer in the asymmetric unit. A water molecule occupies the presumed binding site for the phosphate group of 4-erythrose 4-phosphate Aeropyrum pernix

Inhibitors

Inhibitors Comment Organism Structure
dipicolinic acid 1 mM, 1 h, 21% residual activity Aeropyrum pernix
EDTA 10 mM, 1 h, 21% residual activity Aeropyrum pernix
additional information none of the downstream products of the shikimate biosynthetic pathway tested inhibits the activity of the enzyme Aeropyrum pernix

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.28
-
D-erythrose 4-phosphate pH 7.0, 60°C Aeropyrum pernix
0.891
-
phosphoenolpyruvate pH 7.0, 60°C Aeropyrum pernix

Metals/Ions

Metals/Ions Comment Organism Structure
Zn2+ enzyme contains 0.8 molar equivalents of Zn2+ per subunit Aeropyrum pernix

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
29159
-
2 * 30000, SDS-PAGE, 2 * 29159, calculated Aeropyrum pernix
30000
-
2 * 30000, SDS-PAGE, 2 * 29159, calculated Aeropyrum pernix
55400
-
gel filtration Aeropyrum pernix

Organism

Organism UniProt Comment Textmining
Aeropyrum pernix Q9YEJ7
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant protein Aeropyrum pernix

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
phosphoenolpyruvate + D-erythrose 4-phosphate + H2O
-
Aeropyrum pernix 3-deoxy-D-arabino-hept-2-ulosonate 7-phosphate + phosphate
-
?

Subunits

Subunits Comment Organism
homodimer 2 * 30000, SDS-PAGE, 2 * 29159, calculated Aeropyrum pernix

Synonyms

Synonyms Comment Organism
AroG
-
Aeropyrum pernix
DAH7PS
-
Aeropyrum pernix

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
95
-
-
Aeropyrum pernix

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
90
-
1 h, 50% residual activity Aeropyrum pernix

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1
-
phosphoenolpyruvate pH 7.0, 60°C Aeropyrum pernix

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.7
-
at 60°C Aeropyrum pernix

pH Range

pH Minimum pH Maximum Comment Organism
7
-
60°C, 40% of maximum activity Aeropyrum pernix