Cloned (Comment) | Organism |
---|---|
N-terminally histidine-tagged enzyme is expressed in Escherichia coli KA13 | Mycobacterium tuberculosis |
Crystallization (Comment) | Organism |
---|---|
in complex with chorismate mutase, streak-seeding conditions in 20 mM BTP (1,3-bis[tris(hydroxymethyl)methylamino]propane), pH 7.5, 150 mM NaCl, 0.5 mM TCEP [tris(2-carboxyethyl)phosphine hydrochloride], 0.2 mM phosphoenolpyruvate, crystallization by 0.9 M ammonium sulfate, 100 mM Tris, pH 7.9 to 8.0, and 1 to 5% PEG 400 | Mycobacterium tuberculosis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
phenylalanine | inhibition of the synthase-chorismate mutase complex, based on mutase activity measurements at 30°C, 50 mM BTP (1,3-bis[tris(hydroxymethyl)methylamino]propane), pH 7.5, 0.5 mM TCEP [tris(2-carboxyethyl)phosphine hydrochloride], 0.2 mM phosphoenolpyruvate, and 0.1 mM MnCl2 | Mycobacterium tuberculosis | |
tyrosine | inhibition of the synthase-chorismate mutase complex, based on mutase activity measurements at 30°C, 50 mM BTP (1,3-bis[tris(hydroxymethyl)methylamino]propane), pH 7.5, 0.5 mM TCEP [tris(2-carboxyethyl)phosphine hydrochloride], 0.2 mM phosphoenolpyruvate, and 0.1 mM MnCl2 | Mycobacterium tuberculosis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
51830 | - |
ESI-MS | Mycobacterium tuberculosis |
51830 | - |
expected mass by calculation | Mycobacterium tuberculosis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
phosphoenolpyruvate + erythrose 4-phosphate + H2O | Mycobacterium tuberculosis | - |
3-deoxy-D-arabino-hept-2-ulosonate 7-phosphate + phosphate | - |
? | |
phosphoenolpyruvate + erythrose 4-phosphate + H2O | Mycobacterium tuberculosis H37Rv | - |
3-deoxy-D-arabino-hept-2-ulosonate 7-phosphate + phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycobacterium tuberculosis | O53512 | - |
- |
Mycobacterium tuberculosis H37Rv | O53512 | - |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA affinity chromatography followed by gel filtration, in 20 mM BTP (1,3-bis[tris(hydroxymethyl)methylamino]propane), pH 7.5, 150 mM NaCl, 0.5 mM TCEP [tris(2-carboxyethyl)phosphine hydrochloride], 0.2 mM phosphoenolpyruvate, and 0.1 mM MnCl2, ultrafiltration for concentration of enzyme | Mycobacterium tuberculosis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
phosphoenolpyruvate + erythrose 4-phosphate + H2O | - |
Mycobacterium tuberculosis | 3-deoxy-D-arabino-hept-2-ulosonate 7-phosphate + phosphate | - |
? | |
phosphoenolpyruvate + erythrose 4-phosphate + H2O | - |
Mycobacterium tuberculosis H37Rv | 3-deoxy-D-arabino-hept-2-ulosonate 7-phosphate + phosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
3-deoxy-7-phosphoheptulonate synthase | - |
Mycobacterium tuberculosis |
DAHP synthase | - |
Mycobacterium tuberculosis |
MtDS | - |
Mycobacterium tuberculosis |
General Information | Comment | Organism |
---|---|---|
metabolism | shikimate biosynthetic pathway, noncovalent binding to chorismate mutase enhances the mutase activity more than 100fold, 4 enzyme molecules sandwiched between the chorismate mutase dimers reposition the active site of the mutases | Mycobacterium tuberculosis |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
84 | - |
phosphoenolpyruvate | results within factor two of this otherwise published value, 30°C, 50 mM BTP (1,3-bis[tris(hydroxymethyl)methylamino]propane), pH 7.5 with 100 microM MnCl2, 240 microM D-erythrose-4-phosphate (saturating), less than 10 microM phosphoenolpyruvate, no influence of chorismate mutase concentration on activity | Mycobacterium tuberculosis |