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Literature summary for 2.5.1.54 extracted from

  • Sasso, S.; Okvist, M.; Roderer, K.; Gamper, M.; Codoni, G.; Krengel, U.; Kast, P.
    Structure and function of a complex between chorismate mutase and DAHP synthase: efficiency boost for the junior partner (2009), EMBO J., 28, 2128-2142.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
N-terminally histidine-tagged enzyme is expressed in Escherichia coli KA13 Mycobacterium tuberculosis

Crystallization (Commentary)

Crystallization (Comment) Organism
in complex with chorismate mutase, streak-seeding conditions in 20 mM BTP (1,3-bis[tris(hydroxymethyl)methylamino]propane), pH 7.5, 150 mM NaCl, 0.5 mM TCEP [tris(2-carboxyethyl)phosphine hydrochloride], 0.2 mM phosphoenolpyruvate, crystallization by 0.9 M ammonium sulfate, 100 mM Tris, pH 7.9 to 8.0, and 1 to 5% PEG 400 Mycobacterium tuberculosis

Inhibitors

Inhibitors Comment Organism Structure
phenylalanine inhibition of the synthase-chorismate mutase complex, based on mutase activity measurements at 30°C, 50 mM BTP (1,3-bis[tris(hydroxymethyl)methylamino]propane), pH 7.5, 0.5 mM TCEP [tris(2-carboxyethyl)phosphine hydrochloride], 0.2 mM phosphoenolpyruvate, and 0.1 mM MnCl2 Mycobacterium tuberculosis
tyrosine inhibition of the synthase-chorismate mutase complex, based on mutase activity measurements at 30°C, 50 mM BTP (1,3-bis[tris(hydroxymethyl)methylamino]propane), pH 7.5, 0.5 mM TCEP [tris(2-carboxyethyl)phosphine hydrochloride], 0.2 mM phosphoenolpyruvate, and 0.1 mM MnCl2 Mycobacterium tuberculosis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
51830
-
ESI-MS Mycobacterium tuberculosis
51830
-
expected mass by calculation Mycobacterium tuberculosis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
phosphoenolpyruvate + erythrose 4-phosphate + H2O Mycobacterium tuberculosis
-
3-deoxy-D-arabino-hept-2-ulosonate 7-phosphate + phosphate
-
?
phosphoenolpyruvate + erythrose 4-phosphate + H2O Mycobacterium tuberculosis H37Rv
-
3-deoxy-D-arabino-hept-2-ulosonate 7-phosphate + phosphate
-
?

Organism

Organism UniProt Comment Textmining
Mycobacterium tuberculosis O53512
-
-
Mycobacterium tuberculosis H37Rv O53512
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA affinity chromatography followed by gel filtration, in 20 mM BTP (1,3-bis[tris(hydroxymethyl)methylamino]propane), pH 7.5, 150 mM NaCl, 0.5 mM TCEP [tris(2-carboxyethyl)phosphine hydrochloride], 0.2 mM phosphoenolpyruvate, and 0.1 mM MnCl2, ultrafiltration for concentration of enzyme Mycobacterium tuberculosis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
phosphoenolpyruvate + erythrose 4-phosphate + H2O
-
Mycobacterium tuberculosis 3-deoxy-D-arabino-hept-2-ulosonate 7-phosphate + phosphate
-
?
phosphoenolpyruvate + erythrose 4-phosphate + H2O
-
Mycobacterium tuberculosis H37Rv 3-deoxy-D-arabino-hept-2-ulosonate 7-phosphate + phosphate
-
?

Synonyms

Synonyms Comment Organism
3-deoxy-7-phosphoheptulonate synthase
-
Mycobacterium tuberculosis
DAHP synthase
-
Mycobacterium tuberculosis
MtDS
-
Mycobacterium tuberculosis

General Information

General Information Comment Organism
metabolism shikimate biosynthetic pathway, noncovalent binding to chorismate mutase enhances the mutase activity more than 100fold, 4 enzyme molecules sandwiched between the chorismate mutase dimers reposition the active site of the mutases Mycobacterium tuberculosis

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
84
-
phosphoenolpyruvate results within factor two of this otherwise published value, 30°C, 50 mM BTP (1,3-bis[tris(hydroxymethyl)methylamino]propane), pH 7.5 with 100 microM MnCl2, 240 microM D-erythrose-4-phosphate (saturating), less than 10 microM phosphoenolpyruvate, no influence of chorismate mutase concentration on activity Mycobacterium tuberculosis