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Literature summary for 2.5.1.54 extracted from

  • Okvist, M.; Sasso, S.; Roderer, K.; Kast, P.; Krengel, U.
    A novel noncovalent complex of chorismate mutase and DAHP synthase from Mycobacterium tuberculosis: protein purification, crystallization and X-ray diffraction analysis (2009), Acta Crystallogr. Sect. F, 65, 1048-1052.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expression of enzyme with affinity purification tagwith plasmid pKTDS-HN in Escherichia coli KA13 Mycobacterium tuberculosis

Crystallization (Commentary)

Crystallization (Comment) Organism
in complex with chorismate mutase, hanging-drop method, in 20 mM BTP, pH 7.5, 150 mM NaCl, 0.5 mM tris(2-carboxyethyl)phosphine hydrochloride, 0.2 mM phosphoenolpyruvate and 0.1 mM MnCl2, crystallization after 2 months with no ammonium sulfate and 0.1 M Tris-HCl, pH 7.9-8.0 and PEG 400 or glycerol Mycobacterium tuberculosis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
51830
-
expected mass, calculated from the 472 amino acids Mycobacterium tuberculosis
51830
-
observed by ESI-MS Mycobacterium tuberculosis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
phosphoenolpyruvate + D-erythrose 4-phosphate + H2O Mycobacterium tuberculosis
-
3-deoxy-D-arabino-hept-2-ulosonate 7-phosphate + phosphate
-
?

Organism

Organism UniProt Comment Textmining
Mycobacterium tuberculosis O53512
-
-

Purification (Commentary)

Purification (Comment) Organism
cells centrifuged and resuspended in 20 mM BTP (1,3-bis[tris(hydroxymethyl)methylamino]propane), pH 7.5, 150 mM NaCl, 0.5 mM TCEP (tris(2-carboxyethyl)phosphine hydrochloride), 0.2 mM phosphoenolpyruvate and 0.1 mM MnCl2, cell lysis with lysozyme and sonication, centrifugation, crude extract subjected to Ni-NTA agarose chromatography, elution with 250 mM imidazole in buffer, further purification by preparative FPLC HiLoad 26/60 Superdex 75 gel-filtration column Mycobacterium tuberculosis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
phosphoenolpyruvate + D-erythrose 4-phosphate + H2O
-
Mycobacterium tuberculosis 3-deoxy-D-arabino-hept-2-ulosonate 7-phosphate + phosphate
-
?

Synonyms

Synonyms Comment Organism
3-deoxy-7-phosphoheptulonate synthase
-
Mycobacterium tuberculosis
DAHP synthase
-
Mycobacterium tuberculosis
MtDS
-
Mycobacterium tuberculosis

General Information

General Information Comment Organism
metabolism initial step in the shikimate biosynthesis pathway, enhances the catalytic efficiency of chorismate mutase in a noncovalent enzyme complex Mycobacterium tuberculosis