Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Corynebacterium glutamicum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
EDTA | 22.7% residual activity at 2 mM EDTA for isozyme NCgl0950 DAHP synthase, 49.1% residual activity at 2 mM EDTA for isozyme NCgl2098 DAHP synthase | Corynebacterium glutamicum | |
L-Phe | NCgl0950 DAHP synthase is sensitive to feedback inhibition with 29.1 to 38.8% inhibition at 2 mM, NCgl2098 DAHP synthase is insensitive | Corynebacterium glutamicum | |
L-Trp | NCgl0950 DAHP synthase is sensitive to feedback inhibition, NCgl2098 DAHP synthase is slightly sensitive to feedback inhibition with 10-15% decrease of activity at 5 mM L-Trp | Corynebacterium glutamicum | |
L-Tyr | NCgl0950 DAHP synthase is sensitive to feedback inhibition with 80% loss of activity at 0.03 mM, NCgl2098 DAHP synthase is insensitive, Tyr-linked inhibition is competitive to phophoenolpyruvate | Corynebacterium glutamicum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.16 | - |
phosphoenolpyruvate | isozyme NCgl0950 DAHP synthase, in 50 mM Tris-HCl buffer, pH 7.5, at 30°C | Corynebacterium glutamicum | |
0.29 | - |
D-erythrose 4-phosphate | isozyme NCgl0950 DAHP synthase, in 50 mM Tris-HCl buffer, pH 7.5, at 30°C | Corynebacterium glutamicum | |
2.17 | - |
D-erythrose 4-phosphate | isozyme NCgl2098 DAHP synthase, in 50 mM Tris-HCl buffer, pH 7.5,at 30°C | Corynebacterium glutamicum | |
8.52 | - |
phosphoenolpyruvate | isozyme NCgl2098 DAHP synthase, in 50 mM Tris-HCl buffer, pH 7.5, at 30°C | Corynebacterium glutamicum |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | 90.6% increase of activity at 2 mM for isozyme NCgl0950 DAHP synthase, 31.8% increase of activity at 2 mM for isozyme NCgl2098 DAHP synthase | Corynebacterium glutamicum | |
Mn2+ | 257.5% increase of activity at 2 mM for isozyme NCgl0950 DAHP synthase, 1343.3% increase of activity at 2 mM for isozyme NCgl2098 DAHP synthase | Corynebacterium glutamicum | |
additional information | Mg2+ and Ni2+ are not stimulatory | Corynebacterium glutamicum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
40000 | - |
isozyme NCgl0950 DAHP synthase, SDS-PAGE | Corynebacterium glutamicum |
42000 | - |
2 * 42000, native isozyme NCgl0950 DAHP synthase, gel filtration | Corynebacterium glutamicum |
50000 | - |
2 * 50000, native isozyme NCgl2098 DAHP synthase, gel filtration | Corynebacterium glutamicum |
84000 | - |
native NCgl0950 DAHP synthase, gel filtration | Corynebacterium glutamicum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Corynebacterium glutamicum | - |
strains ATCC 13032 and RES167 | - |
Purification (Comment) | Organism |
---|---|
His-Bind protein chromatography | Corynebacterium glutamicum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
phosphoenolpyruvate + D-erythrose 4-phosphate + H2O | - |
Corynebacterium glutamicum | 2-dehydro-3-deoxy-D-arabino-heptonate 7-phosphate + phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 42000, native isozyme NCgl0950 DAHP synthase, gel filtration | Corynebacterium glutamicum |
homodimer | 2 * 50000, native isozyme NCgl2098 DAHP synthase, gel filtration | Corynebacterium glutamicum |
Synonyms | Comment | Organism |
---|---|---|
DAHP synthase | - |
Corynebacterium glutamicum |
NCgl0950 DAHP synthase | isozyme | Corynebacterium glutamicum |
NCgl2098 DAHP synthase | isozyme | Corynebacterium glutamicum |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.3 | - |
D-erythrose 4-phosphate | isozyme NCgl0950 DAHP synthase, in 50 mM Tris-HCl buffer, pH 7.5, at 30°C | Corynebacterium glutamicum | |
0.35 | - |
phosphoenolpyruvate | isozyme NCgl0950 DAHP synthase, in 50 mM Tris-HCl buffer, pH 7.5, at 30°C | Corynebacterium glutamicum | |
1.14 | - |
D-erythrose 4-phosphate | isozyme NCgl2098 DAHP synthase, in 50 mM Tris-HCl buffer, pH 7.5, at 30°C | Corynebacterium glutamicum | |
1.65 | - |
phosphoenolpyruvate | isozyme NCgl2098 DAHP synthase, in 50 mM Tris-HCl buffer, pH 7.5, at 30°C | Corynebacterium glutamicum |