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Literature summary for 2.5.1.54 extracted from

  • Liu, Y.J.; Li, P.P.; Zhao, K.X.; Wang, B.J.; Jiang, C.Y.; Drake, H.L.; Liu, S.J.
    Corynebacterium glutamicum contains 3-deoxy-D-arabino-heptulosonate 7-phosphate synthases that display novel biochemical features (2008), Appl. Environ. Microbiol., 74, 5497-5503.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Corynebacterium glutamicum

Inhibitors

Inhibitors Comment Organism Structure
EDTA 22.7% residual activity at 2 mM EDTA for isozyme NCgl0950 DAHP synthase, 49.1% residual activity at 2 mM EDTA for isozyme NCgl2098 DAHP synthase Corynebacterium glutamicum
L-Phe NCgl0950 DAHP synthase is sensitive to feedback inhibition with 29.1 to 38.8% inhibition at 2 mM, NCgl2098 DAHP synthase is insensitive Corynebacterium glutamicum
L-Trp NCgl0950 DAHP synthase is sensitive to feedback inhibition, NCgl2098 DAHP synthase is slightly sensitive to feedback inhibition with 10-15% decrease of activity at 5 mM L-Trp Corynebacterium glutamicum
L-Tyr NCgl0950 DAHP synthase is sensitive to feedback inhibition with 80% loss of activity at 0.03 mM, NCgl2098 DAHP synthase is insensitive, Tyr-linked inhibition is competitive to phophoenolpyruvate Corynebacterium glutamicum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.16
-
phosphoenolpyruvate isozyme NCgl0950 DAHP synthase, in 50 mM Tris-HCl buffer, pH 7.5, at 30°C Corynebacterium glutamicum
0.29
-
D-erythrose 4-phosphate isozyme NCgl0950 DAHP synthase, in 50 mM Tris-HCl buffer, pH 7.5, at 30°C Corynebacterium glutamicum
2.17
-
D-erythrose 4-phosphate isozyme NCgl2098 DAHP synthase, in 50 mM Tris-HCl buffer, pH 7.5,at 30°C Corynebacterium glutamicum
8.52
-
phosphoenolpyruvate isozyme NCgl2098 DAHP synthase, in 50 mM Tris-HCl buffer, pH 7.5, at 30°C Corynebacterium glutamicum

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ 90.6% increase of activity at 2 mM for isozyme NCgl0950 DAHP synthase, 31.8% increase of activity at 2 mM for isozyme NCgl2098 DAHP synthase Corynebacterium glutamicum
Mn2+ 257.5% increase of activity at 2 mM for isozyme NCgl0950 DAHP synthase, 1343.3% increase of activity at 2 mM for isozyme NCgl2098 DAHP synthase Corynebacterium glutamicum
additional information Mg2+ and Ni2+ are not stimulatory Corynebacterium glutamicum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
40000
-
isozyme NCgl0950 DAHP synthase, SDS-PAGE Corynebacterium glutamicum
42000
-
2 * 42000, native isozyme NCgl0950 DAHP synthase, gel filtration Corynebacterium glutamicum
50000
-
2 * 50000, native isozyme NCgl2098 DAHP synthase, gel filtration Corynebacterium glutamicum
84000
-
native NCgl0950 DAHP synthase, gel filtration Corynebacterium glutamicum

Organism

Organism UniProt Comment Textmining
Corynebacterium glutamicum
-
strains ATCC 13032 and RES167
-

Purification (Commentary)

Purification (Comment) Organism
His-Bind protein chromatography Corynebacterium glutamicum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
phosphoenolpyruvate + D-erythrose 4-phosphate + H2O
-
Corynebacterium glutamicum 2-dehydro-3-deoxy-D-arabino-heptonate 7-phosphate + phosphate
-
?

Subunits

Subunits Comment Organism
homodimer 2 * 42000, native isozyme NCgl0950 DAHP synthase, gel filtration Corynebacterium glutamicum
homodimer 2 * 50000, native isozyme NCgl2098 DAHP synthase, gel filtration Corynebacterium glutamicum

Synonyms

Synonyms Comment Organism
DAHP synthase
-
Corynebacterium glutamicum
NCgl0950 DAHP synthase isozyme Corynebacterium glutamicum
NCgl2098 DAHP synthase isozyme Corynebacterium glutamicum

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.3
-
D-erythrose 4-phosphate isozyme NCgl0950 DAHP synthase, in 50 mM Tris-HCl buffer, pH 7.5, at 30°C Corynebacterium glutamicum
0.35
-
phosphoenolpyruvate isozyme NCgl0950 DAHP synthase, in 50 mM Tris-HCl buffer, pH 7.5, at 30°C Corynebacterium glutamicum
1.14
-
D-erythrose 4-phosphate isozyme NCgl2098 DAHP synthase, in 50 mM Tris-HCl buffer, pH 7.5, at 30°C Corynebacterium glutamicum
1.65
-
phosphoenolpyruvate isozyme NCgl2098 DAHP synthase, in 50 mM Tris-HCl buffer, pH 7.5, at 30°C Corynebacterium glutamicum