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Literature summary for 2.5.1.54 extracted from

  • Schofield, L.R.; Patchett, M.L.; Parker, E.J.
    Expression, purification, and characterization of 3-deoxy-D-arabino-heptulosonate 7-phosphate synthase from Pyrococcus furiosus (2004), Protein Expr. Purif., 34, 17-27.
    View publication on PubMed

General Stability

General Stability Organism
resistant to denaturation by sodium dodecyl sulfate Pyrococcus furiosus

Inhibitors

Inhibitors Comment Organism Structure
EDTA inactivation, restoration in decreasing order of effectiveness by Zn2+, Cd2+, Mn2+, Co2+, Ni2+, Ca2+, Hg2+, Cu2+ Pyrococcus furiosus
additional information resistant to denaturation by sodium dodecyl sulfate, not inhibitory: phenylalanine, tyrosine, tryptophan Pyrococcus furiosus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.028
-
D-erythrose 4-phosphate 60°C, pH 6.8 Pyrococcus furiosus
0.12
-
phosphoenolpyruvate 60°C, pH 6.8 Pyrococcus furiosus

Metals/Ions

Metals/Ions Comment Organism Structure
Mn2+ assay with 0.1 mM Pyrococcus furiosus
additional information EDTA-inactivated enzyme, restoration in decreasing order of effectiveness by Zn2+, Cd2+, Mn2+, Co2+, Ni2+, Ca2+, Hg2+, Cu2+ Pyrococcus furiosus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
5700
-
gel filtration Pyrococcus furiosus
29224
-
2 * 29226, ESI-MS, 2 * 29224, calculated Pyrococcus furiosus
29226
-
2 * 29226, ESI-MS, 2 * 29224, calculated Pyrococcus furiosus
58450
-
ESI-MS Pyrococcus furiosus

Organism

Organism UniProt Comment Textmining
Pyrococcus furiosus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Pyrococcus furiosus

Renatured (Commentary)

Renatured (Comment) Organism
EDTA-inactivated enzyme, restoration in decreasing order of effectiveness by Zn2+, Cd2+, Mn2+, Co2+, Ni2+, Ca2+, Hg2+, Cu2+ Pyrococcus furiosus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
phosphoenolpyruvate + D-erythrose 4-phosphate + H2O
-
Pyrococcus furiosus 3-deoxy-D-arabino-hept-2-ulosonate 7-phosphate + phosphate
-
?

Subunits

Subunits Comment Organism
dimer 2 * 29226, ESI-MS, 2 * 29224, calculated Pyrococcus furiosus

Synonyms

Synonyms Comment Organism
DAH7PS
-
Pyrococcus furiosus

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
58
-
-
Pyrococcus furiosus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60
-
30 min, presence of phosphoenolpyruvate, stable Pyrococcus furiosus
70
-
30 min, presence of phosphoenolpyruvate, stable Pyrococcus furiosus
95
-
presence of 0.1 mM Mn2+, 130 min, 50% residual activity, absence of Mn2+, 3 min, 50% residual activity Pyrococcus furiosus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.5
-
phosphoenolpyruvate 60°C, pH 6.8 Pyrococcus furiosus