Protein Variants | Comment | Organism |
---|---|---|
DELTA1-15 | N-terminal deletion of amino acids 1-15, no formation of dimeric form | Escherichia coli |
I10A | kinetic parameter similar to wild-type, part of enzyme is monomer instead of dimer | Escherichia coli |
N5K | kinetic parameter similar to wild-type | Escherichia coli |
General Stability | Organism |
---|---|
37°C, 65 min, 50% residual activity | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.028 | - |
phosphoenolpyruvate | mutant N5K, 25°C, pH 6.4 | Escherichia coli | |
0.031 | - |
phosphoenolpyruvate | mutant I10A, 25°C, pH 6.4 | Escherichia coli | |
0.035 | - |
phosphoenolpyruvate | wild-type, 25°C, pH 6.4 | Escherichia coli | |
0.25 | - |
D-erythrose 4-phosphate | wild-type, 25°C, pH 6.4 | Escherichia coli | |
0.278 | - |
D-erythrose 4-phosphate | mutant I10A, 25°C, pH 6.4 | Escherichia coli | |
0.392 | - |
D-erythrose 4-phosphate | mutant N5K, 25°C, pH 6.4 | Escherichia coli | |
12.25 | - |
phosphoenolpyruvate | N-terminal deletion mutant, 25°C, pH 6.4 | Escherichia coli | |
52.36 | - |
D-erythrose 4-phosphate | N-terminal deletion mutant, 25°C, pH 6.4 | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
isoform AroG | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
phosphoenolpyruvate + D-erythrose 4-phosphate + H2O | - |
Escherichia coli | 3-deoxy-D-arabino-hept-2-ulosonate 7-phosphate + phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | wild-type, SDS-PAGE | Escherichia coli |
Synonyms | Comment | Organism |
---|---|---|
AroG | - |
Escherichia coli |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
4.2 | - |
phosphoenolpyruvate | N-terminal deletion mutant, 25°C, pH 6.4 | Escherichia coli | |
56.7 | - |
phosphoenolpyruvate | mutant N5K, 25°C, pH 6.4 | Escherichia coli | |
58.2 | - |
phosphoenolpyruvate | mutant I10A, 25°C, pH 6.4 | Escherichia coli | |
62.3 | - |
phosphoenolpyruvate | wild-type, 25°C, pH 6.4 | Escherichia coli |