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Literature summary for 2.5.1.54 extracted from
- Redox regulation of Arabidopsis 3-deoxy-D-arabino-heptulosonate 7-phosphate synthase (2002), Plant Physiol., 129, 1866-1871.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| dithiothreitol | can partially substitute thioredoxin as activating agent | Arabidopsis thaliana |  |
| reduced thioredoxin | required for activity, can be partially substituted by dithiothreitol | Arabidopsis thaliana | |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression of mature chloroplastic form, without signal sequence, in Escherichia coli | Arabidopsis thaliana |
General Stability
| General Stability | Organism |
|---|---|
| DTT stabilizes the active protein, no influence on the alkylated enzyme | Arabidopsis thaliana |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| iodoacetamide | alkylation, abolishes the dependence on reducing agents | Arabidopsis thaliana | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| chloroplast | chloroplastidic isoenzyme DS-Mn | Arabidopsis thaliana | 9507 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | stimulating, can partly substitute for Mn2+ | Arabidopsis thaliana | |
| Mn2+ | absolutely required, can partly be substituted by Mg2+, but not by Ca2+, Fe2+, and Co2+ | Arabidopsis thaliana | |
| Mn2+ | isozyme DS-Mn | Arabidopsis thaliana | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| phosphoenolpyruvate + D-erythrose 4-phosphate + H2O | Arabidopsis thaliana | first enzyme in the shikimic pathway leading to biosynthesis of aromatic amino acids | 2-dehydro-3-deoxy-D-arabino-heptonate 7-phosphate + phosphate | - |
? | |
| phosphoenolpyruvate + D-erythrose 4-phosphate + H2O | Arabidopsis thaliana | regulatory role of thioredoxin of photosystem I on the chloroplastic enzyme | 2-dehydro-3-deoxy-D-arabino-heptonate 7-phosphate + phosphate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Arabidopsis thaliana | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant from Escherichia coli, mature chloroplastic form, purified to homogeneity | Arabidopsis thaliana |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| phosphoenolpyruvate + D-erythrose 4-phosphate + H2O | - |
Arabidopsis thaliana | 2-dehydro-3-deoxy-D-arabino-heptonate 7-phosphate + phosphate | - |
? | |
| phosphoenolpyruvate + D-erythrose 4-phosphate + H2O | first enzyme in the shikimic pathway leading to biosynthesis of aromatic amino acids | Arabidopsis thaliana | 2-dehydro-3-deoxy-D-arabino-heptonate 7-phosphate + phosphate | - |
? | |
| phosphoenolpyruvate + D-erythrose 4-phosphate + H2O | regulatory role of thioredoxin of photosystem I on the chloroplastic enzyme | Arabidopsis thaliana | 2-dehydro-3-deoxy-D-arabino-heptonate 7-phosphate + phosphate | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Arabidopsis thaliana |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
t1/2: 5 h in absence of DTT | Arabidopsis thaliana |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.6 | - |
assay at | Arabidopsis thaliana |
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