Activating Compound | Comment | Organism | Structure |
---|---|---|---|
dithiothreitol | can partially substitute thioredoxin as activating agent | Arabidopsis thaliana | |
reduced thioredoxin | required for activity, can be partially substituted by dithiothreitol | Arabidopsis thaliana |
Cloned (Comment) | Organism |
---|---|
expression of mature chloroplastic form, without signal sequence, in Escherichia coli | Arabidopsis thaliana |
General Stability | Organism |
---|---|
DTT stabilizes the active protein, no influence on the alkylated enzyme | Arabidopsis thaliana |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
iodoacetamide | alkylation, abolishes the dependence on reducing agents | Arabidopsis thaliana |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
chloroplast | chloroplastidic isoenzyme DS-Mn | Arabidopsis thaliana | 9507 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | stimulating, can partly substitute for Mn2+ | Arabidopsis thaliana | |
Mn2+ | absolutely required, can partly be substituted by Mg2+, but not by Ca2+, Fe2+, and Co2+ | Arabidopsis thaliana | |
Mn2+ | isozyme DS-Mn | Arabidopsis thaliana |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
phosphoenolpyruvate + D-erythrose 4-phosphate + H2O | Arabidopsis thaliana | first enzyme in the shikimic pathway leading to biosynthesis of aromatic amino acids | 2-dehydro-3-deoxy-D-arabino-heptonate 7-phosphate + phosphate | - |
? | |
phosphoenolpyruvate + D-erythrose 4-phosphate + H2O | Arabidopsis thaliana | regulatory role of thioredoxin of photosystem I on the chloroplastic enzyme | 2-dehydro-3-deoxy-D-arabino-heptonate 7-phosphate + phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant from Escherichia coli, mature chloroplastic form, purified to homogeneity | Arabidopsis thaliana |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
phosphoenolpyruvate + D-erythrose 4-phosphate + H2O | - |
Arabidopsis thaliana | 2-dehydro-3-deoxy-D-arabino-heptonate 7-phosphate + phosphate | - |
? | |
phosphoenolpyruvate + D-erythrose 4-phosphate + H2O | first enzyme in the shikimic pathway leading to biosynthesis of aromatic amino acids | Arabidopsis thaliana | 2-dehydro-3-deoxy-D-arabino-heptonate 7-phosphate + phosphate | - |
? | |
phosphoenolpyruvate + D-erythrose 4-phosphate + H2O | regulatory role of thioredoxin of photosystem I on the chloroplastic enzyme | Arabidopsis thaliana | 2-dehydro-3-deoxy-D-arabino-heptonate 7-phosphate + phosphate | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Arabidopsis thaliana |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
t1/2: 5 h in absence of DTT | Arabidopsis thaliana |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.6 | - |
assay at | Arabidopsis thaliana |