Activating Compound | Comment | Organism | Structure |
---|---|---|---|
Phe | slightly activating, mutants S187Y, S187F, S187C | Corynebacterium glutamicum | |
Tyr | mutants S187Y, S187F, S187C | Corynebacterium glutamicum |
Cloned (Comment) | Organism |
---|---|
overexpression of His-tagged wild-type and mutants in Escherichia coli | Corynebacterium glutamicum |
Protein Variants | Comment | Organism |
---|---|---|
S187A | site-directed mutagenesis, slightly reduced activity | Corynebacterium glutamicum |
S187C | site-directed mutagenesis, reduced activity, activation by tyrosine and phenylalanine instead of inhibition like the wild-type | Corynebacterium glutamicum |
S187F | site-directed mutagenesis, highly reduced activity, activation by tyrosine and phenylalanine instead of inhibition like the wild-type | Corynebacterium glutamicum |
S187Y | site-directed mutagenesis, highly reduced activity, activation by tyrosine and phenylalanine instead of inhibition like the wild-type | Corynebacterium glutamicum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Phe | slight inhibition of the wild-type, activation of mutants | Corynebacterium glutamicum | |
Tyr | feedback inhibition; noncompetitive with respect to D-erythrose 4-phosphate, competitive with respect to phosphoenolpyruvate; Tyr-sensitive isozyme; wild-type and mutant S187A | Corynebacterium glutamicum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | mutants | Corynebacterium glutamicum | |
0.0032 | - |
phosphoenolpyruvate | wild-type, pH 7.9, 30°C | Corynebacterium glutamicum | |
0.018 | - |
D-erythrose 4-phosphate | wild-type, pH 7.9, 30°C | Corynebacterium glutamicum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Corynebacterium glutamicum | - |
Tyr-sensitive isozyme | - |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged wild-type and mutants from Escherichia coli | Corynebacterium glutamicum |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
mutants, influence of aromatic amino acids on activity | Corynebacterium glutamicum |
160 | - |
wild-type | Corynebacterium glutamicum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
phosphoenolpyruvate + D-erythrose 4-phosphate + H2O | - |
Corynebacterium glutamicum | 2-dehydro-3-deoxy-D-arabino-heptonate 7-phosphate + phosphate | - |
? |