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Literature summary for 2.5.1.54 extracted from

  • Liao, H.F.; Lin, L.L.; Chien, H.R.; Hsu, W.H.
    Serine 187 is a crucial residue for allosteric regulation of Corynebacterium glutamicum 3-deoxy-D-arabino-heptulosonate-7-phosphate synthase (2001), FEMS Microbiol. Lett., 194, 59-64.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
Phe slightly activating, mutants S187Y, S187F, S187C Corynebacterium glutamicum
Tyr mutants S187Y, S187F, S187C Corynebacterium glutamicum

Cloned(Commentary)

Cloned (Comment) Organism
overexpression of His-tagged wild-type and mutants in Escherichia coli Corynebacterium glutamicum

Protein Variants

Protein Variants Comment Organism
S187A site-directed mutagenesis, slightly reduced activity Corynebacterium glutamicum
S187C site-directed mutagenesis, reduced activity, activation by tyrosine and phenylalanine instead of inhibition like the wild-type Corynebacterium glutamicum
S187F site-directed mutagenesis, highly reduced activity, activation by tyrosine and phenylalanine instead of inhibition like the wild-type Corynebacterium glutamicum
S187Y site-directed mutagenesis, highly reduced activity, activation by tyrosine and phenylalanine instead of inhibition like the wild-type Corynebacterium glutamicum

Inhibitors

Inhibitors Comment Organism Structure
Phe slight inhibition of the wild-type, activation of mutants Corynebacterium glutamicum
Tyr feedback inhibition; noncompetitive with respect to D-erythrose 4-phosphate, competitive with respect to phosphoenolpyruvate; Tyr-sensitive isozyme; wild-type and mutant S187A Corynebacterium glutamicum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information mutants Corynebacterium glutamicum
0.0032
-
phosphoenolpyruvate wild-type, pH 7.9, 30°C Corynebacterium glutamicum
0.018
-
D-erythrose 4-phosphate wild-type, pH 7.9, 30°C Corynebacterium glutamicum

Organism

Organism UniProt Comment Textmining
Corynebacterium glutamicum
-
Tyr-sensitive isozyme
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged wild-type and mutants from Escherichia coli Corynebacterium glutamicum

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
mutants, influence of aromatic amino acids on activity Corynebacterium glutamicum
160
-
wild-type Corynebacterium glutamicum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
phosphoenolpyruvate + D-erythrose 4-phosphate + H2O
-
Corynebacterium glutamicum 2-dehydro-3-deoxy-D-arabino-heptonate 7-phosphate + phosphate
-
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