Cloned (Comment) | Organism |
---|---|
overexpression of His-tagged wild-type and mutants in Escherichia coli | Corynebacterium glutamicum |
Protein Variants | Comment | Organism |
---|---|---|
C145S | site-directed mutagenesis, 16% remaining activity compared to the wild-type, 4.6fold increased Km, 1.6fold decreased kcat, and 13.6fold decreased kcat/Km for phosphoenolpyruvate | Corynebacterium glutamicum |
C334S | site-directed mutagenesis, unaltered properties | Corynebacterium glutamicum |
C67L | site-directed mutagenesis, highly reduced activity, insensitive to inhibition by divalent metal ions | Corynebacterium glutamicum |
C67S | site-directed mutagenesis, inactive | Corynebacterium glutamicum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | no inhibition of mutant C67L; wild-type and mutants | Corynebacterium glutamicum | |
Zn2+ | no inhibition of mutant C67L; wild-type and mutants | Corynebacterium glutamicum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | mutants | Corynebacterium glutamicum | |
0.0043 | - |
phosphoenolpyruvate | recombinant wild-type, 30°C, pH 6.5 | Corynebacterium glutamicum |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | stimulates | Corynebacterium glutamicum | |
Co2+ | wild-type and mutants, best activator | Corynebacterium glutamicum | |
Ni2+ | highly activating | Corynebacterium glutamicum | |
Ni2+ | wild-type and mutants | Corynebacterium glutamicum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Corynebacterium glutamicum | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged wild-type and mutants from Escherichia coli | Corynebacterium glutamicum |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
phosphoenolpyruvate + D-erythrose 4-phosphate + H2O = 3-deoxy-D-arabino-hept-2-ulosonate 7-phosphate + phosphate | Cys67 and Cys145 are important for the catalytic reaction | Corynebacterium glutamicum |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
mutants, influence of aromatic amino acids on activity | Corynebacterium glutamicum |
139.3 | - |
recombinant wild-type | Corynebacterium glutamicum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
phosphoenolpyruvate + D-erythrose 4-phosphate + H2O | - |
Corynebacterium glutamicum | 2-dehydro-3-deoxy-D-arabino-heptonate 7-phosphate + phosphate | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
wild-type and mutants | Corynebacterium glutamicum |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | 50 | wild-type and mutants | Corynebacterium glutamicum |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | mutants | Corynebacterium glutamicum | |
0.0035 | - |
phosphoenolpyruvate | recombinant wild-type, 30°C, pH 6.5 | Corynebacterium glutamicum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
wild-type and mutants | Corynebacterium glutamicum |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
1.5 | 8.5 | wild-type and mutants | Corynebacterium glutamicum |