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Literature summary for 2.5.1.54 extracted from

  • Howe, D.L.; Duewel, H.S.; Woodard, R.W.
    Histidine 268 in 3-deoxy-D-arabino-heptulosonic acid 7-phosphate synthase plays the same role as histidine 202 in 3-deoxy-D-manno-octulosonic acid 8-phosphate synthase (2000), J. Biol. Chem., 275, 40258-40265.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
Phe-sensitive isozyme, wild-type and mutants, expression in Escherichia coli strain BL21(DE3) Escherichia coli

Protein Variants

Protein Variants Comment Organism
C61G site-directed mutagenesis, highly reduced activity, highly increased Km for phosphoenolpyruvate, higher pH-optimum than the wild-type Escherichia coli
H172G site-directed mutagenesis, inactive Escherichia coli
H207G site-directed mutagenesis, reduced activity, increased Km values for the substrates, reduced kcat Escherichia coli
H268G site-directed mutagenesis, inactive Escherichia coli
H304G site-directed mutagenesis, reduced activity, increased Km values for the substrates, increased kcat Escherichia coli
H64G site-directed mutagenesis, reduced activity, increased Km values for the substrates, increased kcat Escherichia coli

Inhibitors

Inhibitors Comment Organism Structure
diethyl dicarbonate Phe-sensitive isozyme, pH-dependent, phosphoenolpyruvate protects wild-type and mutants H64G, H207G, H304G Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.009
-
phosphoenolpyruvate Phe-sensitive isozyme, pH 6.8, 25°C Escherichia coli
0.086
-
D-erythrose 4-phosphate Phe-sensitive isozyme, pH 6.8, 25°C Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
phenylalanine-sensitive isozyme
-

Purification (Commentary)

Purification (Comment) Organism
Phe-sensitive isozyme, recombinant wild-type from overexpressing strain and recombinant mutants Escherichia coli

Reaction

Reaction Comment Organism Reaction ID
phosphoenolpyruvate + D-erythrose 4-phosphate + H2O = 3-deoxy-D-arabino-hept-2-ulosonate 7-phosphate + phosphate His268 is located in the active site Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
phosphoenolpyruvate + D-erythrose 4-phosphate + H2O
-
Escherichia coli 2-dehydro-3-deoxy-D-arabino-heptonate 7-phosphate + phosphate
-
ir

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
assay at Escherichia coli

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
32
-
phosphoenolpyruvate Phe-sensitive isozyme, pH 6.8, 25°C Escherichia coli
32
-
D-erythrose 4-phosphate Phe-sensitive isozyme, pH 6.8, 25°C Escherichia coli

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.8
-
wild-type Phe-sensitive isozyme Escherichia coli
8
-
above, Phe-sensitive iaozyme mutant C61G Escherichia coli