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Literature summary for 2.5.1.54 extracted from

  • Wagner, T.; Shumilin, I.A.; Bauerle, R.; Kretsinger, R.H.
    Structure of 3-deoxy-D-arabino-heptulosonate-7-phosphate synthase from Escherichia coli: Comparison of the Mn2+*2-phosphoglycolate and the Pb2+*2-phosphoenolpyruvate complexes and Implications for catalysis (2000), J. Mol. Biol., 301, 389-399.
    View publication on PubMed

Crystallization (Commentary)

Crystallization (Comment) Organism
Phe-sensitive isozyme, enzyme-Mn2+-2-phosphoglycolate-complexes, hanging drop vapour diffusion method, room temperature, all solutions, except the MnSO4 and the enzyme solution, are treated with Chelex-100 to remove metals, 0.2 mM enzyme subunit solution: 0.37 MnSO4, 4.2 mM 2-phosphoglycolate, 0.1 M Li2SO4, 12% PEG 100 w/v, 20% ethanol v/v, 50 mM 1,3-bis[tris(hydroxy-methyl)methylamino]propane buffer, pH 8.7, reservoir solution: 19% PEG 1000, 0.1 M Li2SO4, 20% ethanol v/v, 50 mM 1,3-bis[tris(hydroxy-methyl)methylamino]propane buffer, X-ray diffraction structure determination and analysis Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli P0AB91 Phe-sensitive isozyme
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
phosphoenolpyruvate + D-erythrose 4-phosphate + H2O
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Escherichia coli 2-dehydro-3-deoxy-D-arabino-heptonate 7-phosphate + phosphate
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Subunits

Subunits Comment Organism
More active site structure Escherichia coli
More subunit structure Escherichia coli