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Literature summary for 2.5.1.54 extracted from

  • Schneider, T.R.; Hartmann, M.; Braus, G.H.
    Crystallization and preliminary X-ray analysis of 3-deoxy-D-arabino-heptulosonate-7-phosphate synthase (tyrosine inhibitable) from Saccharomyces cerevisiae (1999), Acta Crystallogr. Sect. D, 55, 1586-1588.
No PubMed abstract available

Crystallization (Commentary)

Crystallization (Comment) Organism
Tyr-sensitive isozyme, hanging drop vapour diffusion method, protein solution in 1:1 mixture with precipitant, 0.004 ml, + 1 ml precipitant solution: 1. 50 mM KH2PO4, 20% polyethylene glycol 8000 w/v or 2. 0.1 M Tris, pH 9.0, 10 mM NiCl2, 20% polyethylene glycol monomethylester 2000 w/v, room temperature for 3 days, X-ray diffraction structure analysis, usage of cryoprotectant glycerol for investigations Saccharomyces cerevisiae

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
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-
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Saccharomyces cerevisiae
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ARO4-encoded isozyme is a tyrosine-sensitive isozyme
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Saccharomyces cerevisiae RH1326
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-
-

Purification (Commentary)

Purification (Comment) Organism
Tyr-sensitive isozyme Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
phosphoenolpyruvate + D-erythrose 4-phosphate + H2O
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Saccharomyces cerevisiae 2-dehydro-3-deoxy-D-arabino-heptonate 7-phosphate + phosphate
-
?
phosphoenolpyruvate + D-erythrose 4-phosphate + H2O
-
Saccharomyces cerevisiae RH1326 2-dehydro-3-deoxy-D-arabino-heptonate 7-phosphate + phosphate
-
?