Crystallization (Comment) | Organism |
---|---|
Tyr-sensitive isozyme, hanging drop vapour diffusion method, protein solution in 1:1 mixture with precipitant, 0.004 ml, + 1 ml precipitant solution: 1. 50 mM KH2PO4, 20% polyethylene glycol 8000 w/v or 2. 0.1 M Tris, pH 9.0, 10 mM NiCl2, 20% polyethylene glycol monomethylester 2000 w/v, room temperature for 3 days, X-ray diffraction structure analysis, usage of cryoprotectant glycerol for investigations | Saccharomyces cerevisiae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | - |
- |
- |
Saccharomyces cerevisiae | - |
ARO4-encoded isozyme is a tyrosine-sensitive isozyme | - |
Saccharomyces cerevisiae RH1326 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Tyr-sensitive isozyme | Saccharomyces cerevisiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
phosphoenolpyruvate + D-erythrose 4-phosphate + H2O | - |
Saccharomyces cerevisiae | 2-dehydro-3-deoxy-D-arabino-heptonate 7-phosphate + phosphate | - |
? | |
phosphoenolpyruvate + D-erythrose 4-phosphate + H2O | - |
Saccharomyces cerevisiae RH1326 | 2-dehydro-3-deoxy-D-arabino-heptonate 7-phosphate + phosphate | - |
? |