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Literature summary for 2.5.1.54 extracted from

  • Walker, G.E.; Dunbar, B.; Hunter, I.S.; Nimmo, H.G.; Coggins, J.R.
    Evidence for a novel class of microbial 3-deoxy-D-arabino-heptulosonate-7-phosphate synthase in Streptomyces rimosus and Neurospora crassa (1996), Microbiology, 142, 1973-1982.
No PubMed abstract available

General Stability

General Stability Organism
overnight dialysis against buffer containing 10 mM EDTA at 4°C results in 85% inactivation Streptomyces coelicolor

Inhibitors

Inhibitors Comment Organism Structure
EDTA
-
Neurospora crassa
EDTA
-
Streptomyces coelicolor
Trp Trp-sensitive isozyme Neurospora crassa
Trp Trp-sensitive isozyme Streptomyces coelicolor
Trp Trp-sensitive isozyme Streptomyces rimosus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.092
-
phosphoenolpyruvate Trp-sensitive isozyme, pH 7.0, 30°C Streptomyces coelicolor
0.195
-
D-erythrose 4-phosphate Trp-sensitive isozyme, pH 7.0, 30°C Streptomyces coelicolor

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
54000
-
2 * 54000, SDS-PAGE Neurospora crassa
54000
-
2 * 54000, SDS-PAGE Streptomyces coelicolor
54000
-
2 * 54000, SDS-PAGE Streptomyces rimosus
107000
-
Trp-sensitive isozyme, gel filtration Streptomyces coelicolor

Organism

Organism UniProt Comment Textmining
Neurospora crassa
-
tryptophan-sensitive isozyme
-
Streptomyces coelicolor
-
-
-
Streptomyces coelicolor
-
Trp-sensitive isozyme
-
Streptomyces coelicolor A3(2)
-
-
-
Streptomyces rimosus
-
Trp-sensitive isozyme
-

Purification (Commentary)

Purification (Comment) Organism
Trp-sensitive isozyme to homogeneity, 2000fold Neurospora crassa
Trp-sensitive isozyme to homogeneity, 2380fold, large scale Streptomyces coelicolor
Trp-sensitive isozyme to homogeneity, 3750fold Streptomyces rimosus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
7.7
-
purified Trp-sensitive isozyme Neurospora crassa
8.33
-
purified Trp-sensitive isozyme Streptomyces coelicolor
11.02
-
purified Trp-sensitive isozyme Streptomyces rimosus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
phosphoenolpyruvate + D-erythrose 4-phosphate + H2O
-
Neurospora crassa 2-dehydro-3-deoxy-D-arabino-heptonate 7-phosphate + phosphate
-
?
phosphoenolpyruvate + D-erythrose 4-phosphate + H2O
-
Streptomyces coelicolor 2-dehydro-3-deoxy-D-arabino-heptonate 7-phosphate + phosphate
-
?
phosphoenolpyruvate + D-erythrose 4-phosphate + H2O
-
Streptomyces rimosus 2-dehydro-3-deoxy-D-arabino-heptonate 7-phosphate + phosphate
-
?
phosphoenolpyruvate + D-erythrose 4-phosphate + H2O
-
Streptomyces coelicolor A3(2) 2-dehydro-3-deoxy-D-arabino-heptonate 7-phosphate + phosphate
-
?

Subunits

Subunits Comment Organism
dimer 2 * 54000, SDS-PAGE Neurospora crassa
dimer 2 * 54000, SDS-PAGE Streptomyces coelicolor
dimer 2 * 54000, SDS-PAGE Streptomyces rimosus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
assay at Streptomyces coelicolor

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
assay at Streptomyces coelicolor
7
-
assay at Streptomyces rimosus
7 7.4 Trp-sensitive isozyme Streptomyces coelicolor