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Literature summary for 2.5.1.54 extracted from

  • Euverink, G.J.W.; Hessels, G.I.; Franke, C.; Dijkhuizen, L.
    Chorismate mutase and 3-deoxy-D-arabino-heptulosonate 7-phosphate synthase of the methylotrophic actinomycete Amycolatopsis methanolica (1995), Appl. Environ. Microbiol., 61, 3796-3803.
    View publication on PubMedView publication on EuropePMC

Inhibitors

Inhibitors Comment Organism Structure
Phe DSI; Phe-sensitive isozyme Amycolatopsis methanolica
Trp DSI; Trp-sensitive isozyme Amycolatopsis methanolica
Tyr DSI and DSII; Tyr-sensitive isozyme Amycolatopsis methanolica

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.4
-
phosphoenolpyruvate isozyme DS II, pH 7.5, 37°C Amycolatopsis methanolica
1.1
-
D-erythrose 4-phosphate DS II isozyme, pH 7.5, 37°C Amycolatopsis methanolica

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
phosphoenolpyruvate + D-erythrose 4-phosphate + H2O Amycolatopsis methanolica key regulatory enzyme in L-Phe and L-Tyr biosynthesis 2-dehydro-3-deoxy-D-arabino-heptonate 7-phosphate + phosphate
-
?

Organism

Organism UniProt Comment Textmining
Amycolatopsis methanolica
-
wild type strain WV2 contains DS I, a leaky L-Phe-requiring auxotroph mutant strain GH141 grown under L-Phe limitation possesses additional DS II activity
-

Purification (Commentary)

Purification (Comment) Organism
-
Amycolatopsis methanolica

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
phosphoenolpyruvate + D-erythrose 4-phosphate + H2O
-
Amycolatopsis methanolica 2-dehydro-3-deoxy-D-arabino-heptonate 7-phosphate + phosphate
-
?
phosphoenolpyruvate + D-erythrose 4-phosphate + H2O key regulatory enzyme in L-Phe and L-Tyr biosynthesis Amycolatopsis methanolica 2-dehydro-3-deoxy-D-arabino-heptonate 7-phosphate + phosphate
-
?