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Literature summary for 2.5.1.54 extracted from

  • Ramilo, C.A.; Evans, J.N.S.
    Overexpression, purification, and characterization of tyrosine-sensitive 3-deoxy-D-arabino-heptulosonic acid 7-phosphate synthase from Escherichia coli (1997), Protein Expr. Purif., 9, 253-261.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
gene aroF, Tyr-sensitive isozyme, overexpression in strain BL21(DE3) Escherichia coli

Inhibitors

Inhibitors Comment Organism Structure
CN- Tyr-sensitive isozyme, strong inhibition, reactivation by divalent cations only to a small extent Escherichia coli
EDTA Mn2+, Cd2+, Co2+, Fe2+, Cu2+, Mg2+ or Zn2+ reactivate, Fe2+ and Cu2+ only partially reactivate Escherichia coli
Tyr Tyr-sensitive isozyme Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information kinetics Escherichia coli
additional information
-
additional information pH-dependence Escherichia coli
0.013
-
phosphoenolpyruvate Tyr-sensitive isozyme, 25°C Escherichia coli
0.0814
-
D-erythrose 4-phosphate Tyr-sensitive isozyme, 25°C Escherichia coli

Metals/Ions

Metals/Ions Comment Organism Structure
Cd2+ activates, Tyr-sensitive isozyme Escherichia coli
Cu2+ activates, Tyr-sensitive isozyme Escherichia coli
Fe2+ activates, Tyr-sensitive isozyme Escherichia coli
Mg2+ activates, Tyr-sensitive isozyme Escherichia coli
Mn2+ Tyr-sensitive isozyme, best activator Escherichia coli
Zn2+ activates, Tyr-sensitive isozyme Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
tyrosine-sensitive isozyme
-

Purification (Commentary)

Purification (Comment) Organism
2fold Escherichia coli
recombinant from overexpressing strain Escherichia coli

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
Escherichia coli
22
-
purified recombinant Tyr-sensitive isozyme, with Cd2+ Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
phosphoenolpyruvate + D-erythrose 4-phosphate + H2O
-
Escherichia coli 2-dehydro-3-deoxy-D-arabino-heptonate 7-phosphate + phosphate
-
?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
29.5
-
phosphoenolpyruvate
-
Escherichia coli

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
additional information
-
Tyr-sensitive isozyme, pIs: 6.1 and 8.9 Escherichia coli
7 7.5 recombinant Tyr-sensitive isozyme Escherichia coli