Inhibitors | Comment | Organism | Structure |
---|---|---|---|
EDTA | Co2+, Mn2+, Zn2+ and Fe2+ restore activity; phosphoenolpyruvate, 2 mM, partially protects from inactivation, does not restore activity | Saccharomyces cerevisiae | |
Phe | competitive with respect to D-erythrose 4-phosphate and non-competitive to phosphoenolpyruvate; Phe-sensitive isozyme | Saccharomyces cerevisiae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.018 | - |
phosphoenolpyruvate | Phe-sensitive isozyme, pH 6.8, 30°C | Saccharomyces cerevisiae | |
0.13 | - |
D-erythrose 4-phosphate | Phe-sensitive isozyme, pH 6.8, 30°C | Saccharomyces cerevisiae |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | contains 0.6 mol of iron per mol of enzyme | Saccharomyces cerevisiae |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
42130 | - |
1 * 42130, calculation from nucleotide sequence | Saccharomyces cerevisiae |
53000 | - |
gel filtration | Saccharomyces cerevisiae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | - |
Phe-sensitive isozyme | - |
Purification (Comment) | Organism |
---|---|
Phe-sensitive isozyme, 11fold to homogeneity from overproducing strain | Saccharomyces cerevisiae |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Saccharomyces cerevisiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
phosphoenolpyruvate + D-erythrose 4-phosphate + H2O | - |
Saccharomyces cerevisiae | 2-dehydro-3-deoxy-D-arabino-heptonate 7-phosphate + phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 42130, calculation from nucleotide sequence | Saccharomyces cerevisiae |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Saccharomyces cerevisiae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.8 | - |
- |
Saccharomyces cerevisiae |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.01 | - |
Phe | Phe-sensitive isozyme, pH 6.8, 30°C | Saccharomyces cerevisiae |