Cloned (Comment) | Organism |
---|---|
ARO4 gene, tyrosine-sensitive isozyme, overexpression from plasmid in strain RH1326 | Saccharomyces cerevisiae |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
EDTA | Co2+, Zn2+, Cu2+, and Fe3+ restore activity | Saccharomyces cerevisiae | |
Phe | competitive with respect to D-erythrose 4-phosphate and non-competitive to phosphoenolpyruvate; Tyr-sensitive isozyme is less inhibited than the Phe-sensitive isozyme | Saccharomyces cerevisiae | |
Tyr | noncompetitive with respect to D-erythrose 4-phosphate, competitive with respect to phosphoenolpyruvate; Tyr-sensitive isozyme | Saccharomyces cerevisiae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.125 | - |
phosphoenolpyruvate | Tyr-sensitive isozyme, pH 6.8 | Saccharomyces cerevisiae | |
0.5 | - |
D-erythrose 4-phosphate | Tyr-sensitive isozyme, pH 6.8 | Saccharomyces cerevisiae |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | Tyr-sensitive isozyme, metal factor required | Saccharomyces cerevisiae |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
42000 | - |
x * 42000, Tyr-sensitive isozyme, SDS-PAGE | Saccharomyces cerevisiae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | - |
ARO3-encoded isozyme is a phenylalanine-sensitive isozyme | - |
Saccharomyces cerevisiae | - |
ARO4-encoded isozyme is a tyrosine-sensitive isozyme | - |
Purification (Comment) | Organism |
---|---|
recombinantly overexpresed Tyr-sensitive isozyme, 7.5fold | Saccharomyces cerevisiae |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
phosphoenolpyruvate + D-erythrose 4-phosphate + H2O = 3-deoxy-D-arabino-hept-2-ulosonate 7-phosphate + phosphate | sequential mechanism | Saccharomyces cerevisiae |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
8.24 | - |
purified recombinant Tyr-sensitive isozyme | Saccharomyces cerevisiae |
Storage Stability | Organism |
---|---|
-20°C, 50% glycerol, stable for at least 4 weeks | Saccharomyces cerevisiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
phosphoenolpyruvate + D-erythrose 4-phosphate + H2O | - |
Saccharomyces cerevisiae | 2-dehydro-3-deoxy-D-arabino-heptonate 7-phosphate + phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 42000, Tyr-sensitive isozyme, SDS-PAGE | Saccharomyces cerevisiae |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.5 | 7 | - |
Saccharomyces cerevisiae |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0009 | - |
Tyr | Tyr-sensitive isozyme, pH 6.8 | Saccharomyces cerevisiae | |
0.27 | - |
Phe | Tyr-sensitive isozyme, pH 6.8 | Saccharomyces cerevisiae |