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Literature summary for 2.5.1.54 extracted from

  • Schnappauf, G.; Hartmann, M.; Künzler, M.; Braus, G.H.
    The two 3-deoxy-D-arabino-heptulosonate-7-phosphate synthase isoenzymes from Saccharomyces cerevisiae show different kinetic modes of inhibition (1998), Arch. Microbiol., 169, 517-524.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
ARO4 gene, tyrosine-sensitive isozyme, overexpression from plasmid in strain RH1326 Saccharomyces cerevisiae

Inhibitors

Inhibitors Comment Organism Structure
EDTA Co2+, Zn2+, Cu2+, and Fe3+ restore activity Saccharomyces cerevisiae
Phe competitive with respect to D-erythrose 4-phosphate and non-competitive to phosphoenolpyruvate; Tyr-sensitive isozyme is less inhibited than the Phe-sensitive isozyme Saccharomyces cerevisiae
Tyr noncompetitive with respect to D-erythrose 4-phosphate, competitive with respect to phosphoenolpyruvate; Tyr-sensitive isozyme Saccharomyces cerevisiae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.125
-
phosphoenolpyruvate Tyr-sensitive isozyme, pH 6.8 Saccharomyces cerevisiae
0.5
-
D-erythrose 4-phosphate Tyr-sensitive isozyme, pH 6.8 Saccharomyces cerevisiae

Metals/Ions

Metals/Ions Comment Organism Structure
additional information Tyr-sensitive isozyme, metal factor required Saccharomyces cerevisiae

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
42000
-
x * 42000, Tyr-sensitive isozyme, SDS-PAGE Saccharomyces cerevisiae

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
-
ARO3-encoded isozyme is a phenylalanine-sensitive isozyme
-
Saccharomyces cerevisiae
-
ARO4-encoded isozyme is a tyrosine-sensitive isozyme
-

Purification (Commentary)

Purification (Comment) Organism
recombinantly overexpresed Tyr-sensitive isozyme, 7.5fold Saccharomyces cerevisiae

Reaction

Reaction Comment Organism Reaction ID
phosphoenolpyruvate + D-erythrose 4-phosphate + H2O = 3-deoxy-D-arabino-hept-2-ulosonate 7-phosphate + phosphate sequential mechanism Saccharomyces cerevisiae

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
8.24
-
purified recombinant Tyr-sensitive isozyme Saccharomyces cerevisiae

Storage Stability

Storage Stability Organism
-20°C, 50% glycerol, stable for at least 4 weeks Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
phosphoenolpyruvate + D-erythrose 4-phosphate + H2O
-
Saccharomyces cerevisiae 2-dehydro-3-deoxy-D-arabino-heptonate 7-phosphate + phosphate
-
?

Subunits

Subunits Comment Organism
? x * 42000, Tyr-sensitive isozyme, SDS-PAGE Saccharomyces cerevisiae

pH Range

pH Minimum pH Maximum Comment Organism
6.5 7
-
Saccharomyces cerevisiae

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.0009
-
Tyr Tyr-sensitive isozyme, pH 6.8 Saccharomyces cerevisiae
0.27
-
Phe Tyr-sensitive isozyme, pH 6.8 Saccharomyces cerevisiae