Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.5.1.54 extracted from

  • Berry, A.; Johnson, J.L.; Jensen, R.A.
    Phenylalanine hydroxylase and isozymes of 3-deoxy-D-arabino-heptulosonate 7-phosphate synthase in relationship to the phylogenetic position of Pseudomonas acidovorans (Ps. Sp. ATCC 11299a) (1985), Arch. Microbiol., 141, 32-39.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
Phe 0.04 mM, 50% inhibition of DAHP synthase-phe; L-Phe; Phe-sensitive isozyme Delftia acidovorans
Tyr 0.04 mM, 50% inhibition of DAHP synthase-tyr; L-Tyr; Tyr-sensitive isozyme Delftia acidovorans

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.2
-
phosphoenolpyruvate DAHP synthase-phe, pH 7.0, 37°C Delftia acidovorans
0.26
-
phosphoenolpyruvate DAHP synthase-tyr, pH 7.0, 37°C Delftia acidovorans
0.77
-
D-erythrose 4-phosphate DAHP synthase-tyr, pH 7.0, 37°C Delftia acidovorans
0.87
-
D-erythrose 4-phosphate DAHP synthase-phe, pH 7.0, 37°C Delftia acidovorans

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
67000
-
DAHP synthase-phe, gel filtration Delftia acidovorans
251000
-
DAHP synthase-tyr, gel filtration Delftia acidovorans

Organism

Organism UniProt Comment Textmining
Comamonas testosteroni
-
strain ATCC 17409
-
Delftia acidovorans
-
strain ATCC 11299a, two distinct regulatory isoenzymes: DAHP synthase-phe and DAHP synthase-tyr
-
Delftia acidovorans
-
i.e. Comamonas terrigena
-
Hydrogenophaga palleronii
-
strain ATCC 17724
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
phosphoenolpyruvate + D-erythrose 4-phosphate + H2O
-
Comamonas testosteroni 2-dehydro-3-deoxy-D-arabino-heptonate 7-phosphate + phosphate
-
?
phosphoenolpyruvate + D-erythrose 4-phosphate + H2O
-
Delftia acidovorans 2-dehydro-3-deoxy-D-arabino-heptonate 7-phosphate + phosphate
-
?
phosphoenolpyruvate + D-erythrose 4-phosphate + H2O
-
Hydrogenophaga palleronii 2-dehydro-3-deoxy-D-arabino-heptonate 7-phosphate + phosphate
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Delftia acidovorans

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.8 7.2 DAHP synthase-phe Delftia acidovorans
7
-
DAHP synthase-tyr Delftia acidovorans