Activating Compound | Comment | Organism | Structure |
---|---|---|---|
L-Trp | activates | Daucus carota |
General Stability | Organism |
---|---|
Mn2+ stabilizes during purification | Daucus carota |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
7-phospho-2-dehydro-3-deoxy-D-arabino-heptonate | product inhibition | Daucus carota | |
EDTA | - |
Daucus carota | |
metal chelators | - |
Daucus carota | |
phosphate | non-competitive with respect to both phosphoenolpyruvate and D-erythrose 4-phosphate | Daucus carota |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.03 | - |
phosphoenolpyruvate | pH 7.0, 25°C | Daucus carota | |
0.07 | - |
D-erythrose 4-phosphate | pH 7.0, 25°C | Daucus carota |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mn2+ | stimulates | Daucus carota |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
53000 | - |
2 * 53000, SDS-PAGE | Daucus carota |
103000 | - |
gel filtration | Daucus carota |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
phosphoenolpyruvate + D-erythrose 4-phosphate + H2O | Daucus carota | first enzyme of the aromatic amino acid biosynthesis | 2-dehydro-3-deoxy-D-arabino-heptonate 7-phosphate + phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Daucus carota | - |
3 enzymes: I, II, and II | - |
Purification (Comment) | Organism |
---|---|
enzyme III | Daucus carota |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
root | - |
Daucus carota | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
7.1 | - |
purified enzyme | Daucus carota |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
phosphoenolpyruvate + D-erythrose 4-phosphate + H2O | - |
Daucus carota | 2-dehydro-3-deoxy-D-arabino-heptonate 7-phosphate + phosphate | - |
? | |
phosphoenolpyruvate + D-erythrose 4-phosphate + H2O | first enzyme of the aromatic amino acid biosynthesis | Daucus carota | 2-dehydro-3-deoxy-D-arabino-heptonate 7-phosphate + phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 53000, SDS-PAGE | Daucus carota |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Daucus carota |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
assay at | Daucus carota |
7.1 | - |
- |
Daucus carota |