General Stability | Organism |
---|---|
loss of 50% activity by freezing and thawing the cell extract | Cupriavidus necator |
unstable during manipulations such as dialysis, dilution, ammonium sulfate fractionation, chromatography on DEAE-cellulose or Sephadex G-200 | Cupriavidus necator |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Co2+ | weak | Cupriavidus necator | |
Cu2+ | weak | Cupriavidus necator | |
EDTA | no inhibition | Cupriavidus necator | |
Hg2+ | complete inhibition at 1 mM | Cupriavidus necator | |
additional information | no inhibition by Tyr and Phe | Xanthobacter autotrophicus | |
Na2SO4 | slightly | Cupriavidus necator | |
Ni2+ | weak | Cupriavidus necator | |
Phe | 0.5 mM, 60% inhibition; Phe-sensitive isozyme | Cupriavidus necator | |
Phe | no inhibition; Phe-sensitive isozyme; strain 12/60/X, no inhibition; strain H1, strain H20 and strain 3/2: cumulative inhibition of Tyr and Phe | Xanthobacter autotrophicus | |
Tyr | 0.5 mM, 20% inhibition; Tyr-sensitive isozyme | Cupriavidus necator | |
Tyr | strain 33/X strongly but not totally inhibited; strain H1, strain H20 and strain 3/2: cumulative inhibition of Tyr and Phe; Tyr-sensitive isozyme | Xanthobacter autotrophicus | |
Zn2+ | - |
Cupriavidus necator |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.043 | - |
phosphoenolpyruvate | pH 7.8, 25°C | Cupriavidus necator | |
0.055 | - |
D-erythrose 4-phosphate | pH 7.8, 25°C | Cupriavidus necator |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
phosphoenolpyruvate + D-erythrose 4-phosphate + H2O | Cupriavidus necator | no repression by the aromatic amino acids | 2-dehydro-3-deoxy-D-arabino-heptonate 7-phosphate + phosphate | - |
? | |
phosphoenolpyruvate + D-erythrose 4-phosphate + H2O | Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1 | no repression by the aromatic amino acids | 2-dehydro-3-deoxy-D-arabino-heptonate 7-phosphate + phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Alcaligenes faecalis | - |
- |
- |
Cupriavidus necator | - |
- |
- |
Cupriavidus necator | - |
basonym Alcaligenes eutrophus | - |
Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1 | - |
- |
- |
Xanthobacter autotrophicus | - |
- |
- |
Xanthobacter autotrophicus 7C | - |
- |
- |
Purification (Comment) | Organism |
---|---|
partially | Cupriavidus necator |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.026 | - |
partially purified enzyme | Cupriavidus necator |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
phosphoenolpyruvate + D-erythrose 4-phosphate + H2O | - |
Alcaligenes faecalis | 2-dehydro-3-deoxy-D-arabino-heptonate 7-phosphate + phosphate | - |
? | |
phosphoenolpyruvate + D-erythrose 4-phosphate + H2O | - |
Cupriavidus necator | 2-dehydro-3-deoxy-D-arabino-heptonate 7-phosphate + phosphate | - |
? | |
phosphoenolpyruvate + D-erythrose 4-phosphate + H2O | - |
Xanthobacter autotrophicus | 2-dehydro-3-deoxy-D-arabino-heptonate 7-phosphate + phosphate | - |
? | |
phosphoenolpyruvate + D-erythrose 4-phosphate + H2O | no repression by the aromatic amino acids | Cupriavidus necator | 2-dehydro-3-deoxy-D-arabino-heptonate 7-phosphate + phosphate | - |
? | |
phosphoenolpyruvate + D-erythrose 4-phosphate + H2O | - |
Xanthobacter autotrophicus 7C | 2-dehydro-3-deoxy-D-arabino-heptonate 7-phosphate + phosphate | - |
? | |
phosphoenolpyruvate + D-erythrose 4-phosphate + H2O | - |
Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1 | 2-dehydro-3-deoxy-D-arabino-heptonate 7-phosphate + phosphate | - |
? | |
phosphoenolpyruvate + D-erythrose 4-phosphate + H2O | no repression by the aromatic amino acids | Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1 | 2-dehydro-3-deoxy-D-arabino-heptonate 7-phosphate + phosphate | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Cupriavidus necator |
40 | - |
- |
Cupriavidus necator |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
phosphoenolpyruvate protects against inactivation | Cupriavidus necator |
50 | - |
inactivation above | Cupriavidus necator |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.2 | 8.2 | - |
Cupriavidus necator |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.5 | 9 | pH 6.5: about 35% of maximal activity, pH 9.0: about 60% of maximal activity | Cupriavidus necator |