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Literature summary for 2.5.1.54 extracted from

  • Friedrich, C.G.; Schlegel, H.G.
    Aromatic amino acid biosynthesis in Alcaligenes eutrophus H16. I. Properties and regulation of 3-deoxy-D-arabino heptulosonate 7-phosphate synthase (1975), Arch. Microbiol., 103, 133-140.
    View publication on PubMed

General Stability

General Stability Organism
loss of 50% activity by freezing and thawing the cell extract Cupriavidus necator
unstable during manipulations such as dialysis, dilution, ammonium sulfate fractionation, chromatography on DEAE-cellulose or Sephadex G-200 Cupriavidus necator

Inhibitors

Inhibitors Comment Organism Structure
Co2+ weak Cupriavidus necator
Cu2+ weak Cupriavidus necator
EDTA no inhibition Cupriavidus necator
Hg2+ complete inhibition at 1 mM Cupriavidus necator
additional information no inhibition by Tyr and Phe Xanthobacter autotrophicus
Na2SO4 slightly Cupriavidus necator
Ni2+ weak Cupriavidus necator
Phe 0.5 mM, 60% inhibition; Phe-sensitive isozyme Cupriavidus necator
Phe no inhibition; Phe-sensitive isozyme; strain 12/60/X, no inhibition; strain H1, strain H20 and strain 3/2: cumulative inhibition of Tyr and Phe Xanthobacter autotrophicus
Tyr 0.5 mM, 20% inhibition; Tyr-sensitive isozyme Cupriavidus necator
Tyr strain 33/X strongly but not totally inhibited; strain H1, strain H20 and strain 3/2: cumulative inhibition of Tyr and Phe; Tyr-sensitive isozyme Xanthobacter autotrophicus
Zn2+
-
Cupriavidus necator

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.043
-
phosphoenolpyruvate pH 7.8, 25°C Cupriavidus necator
0.055
-
D-erythrose 4-phosphate pH 7.8, 25°C Cupriavidus necator

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
phosphoenolpyruvate + D-erythrose 4-phosphate + H2O Cupriavidus necator no repression by the aromatic amino acids 2-dehydro-3-deoxy-D-arabino-heptonate 7-phosphate + phosphate
-
?
phosphoenolpyruvate + D-erythrose 4-phosphate + H2O Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1 no repression by the aromatic amino acids 2-dehydro-3-deoxy-D-arabino-heptonate 7-phosphate + phosphate
-
?

Organism

Organism UniProt Comment Textmining
Alcaligenes faecalis
-
-
-
Cupriavidus necator
-
-
-
Cupriavidus necator
-
basonym Alcaligenes eutrophus
-
Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1
-
-
-
Xanthobacter autotrophicus
-
-
-
Xanthobacter autotrophicus 7C
-
-
-

Purification (Commentary)

Purification (Comment) Organism
partially Cupriavidus necator

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.026
-
partially purified enzyme Cupriavidus necator

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
phosphoenolpyruvate + D-erythrose 4-phosphate + H2O
-
Alcaligenes faecalis 2-dehydro-3-deoxy-D-arabino-heptonate 7-phosphate + phosphate
-
?
phosphoenolpyruvate + D-erythrose 4-phosphate + H2O
-
Cupriavidus necator 2-dehydro-3-deoxy-D-arabino-heptonate 7-phosphate + phosphate
-
?
phosphoenolpyruvate + D-erythrose 4-phosphate + H2O
-
Xanthobacter autotrophicus 2-dehydro-3-deoxy-D-arabino-heptonate 7-phosphate + phosphate
-
?
phosphoenolpyruvate + D-erythrose 4-phosphate + H2O no repression by the aromatic amino acids Cupriavidus necator 2-dehydro-3-deoxy-D-arabino-heptonate 7-phosphate + phosphate
-
?
phosphoenolpyruvate + D-erythrose 4-phosphate + H2O
-
Xanthobacter autotrophicus 7C 2-dehydro-3-deoxy-D-arabino-heptonate 7-phosphate + phosphate
-
?
phosphoenolpyruvate + D-erythrose 4-phosphate + H2O
-
Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1 2-dehydro-3-deoxy-D-arabino-heptonate 7-phosphate + phosphate
-
?
phosphoenolpyruvate + D-erythrose 4-phosphate + H2O no repression by the aromatic amino acids Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1 2-dehydro-3-deoxy-D-arabino-heptonate 7-phosphate + phosphate
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
assay at Cupriavidus necator
40
-
-
Cupriavidus necator

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
additional information
-
phosphoenolpyruvate protects against inactivation Cupriavidus necator
50
-
inactivation above Cupriavidus necator

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.2 8.2
-
Cupriavidus necator

pH Range

pH Minimum pH Maximum Comment Organism
6.5 9 pH 6.5: about 35% of maximal activity, pH 9.0: about 60% of maximal activity Cupriavidus necator