Activating Compound | Comment | Organism | Structure |
---|---|---|---|
dithiothreitol | stimulates | Kitasatospora aureofaciens |
General Stability | Organism |
---|---|
dithiothreitol stabilizes during incubation at 30°C for 15 min | Kitasatospora aureofaciens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
EDTA | reversible by Co2+ | Kitasatospora aureofaciens | |
p-hydroxymercuribenzoate | - |
Kitasatospora aureofaciens | |
Phe | no inhibition | Kitasatospora aureofaciens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.16 | - |
D-erythrose 4-phosphate | 30°C, pH 7.0 | Kitasatospora aureofaciens | |
0.3 | - |
phosphoenolpyruvate | 30°C, pH 7.0 | Kitasatospora aureofaciens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | stimulates | Kitasatospora aureofaciens | |
Co2+ | required for full activity | Kitasatospora aureofaciens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
97000 | - |
gel filtration | Kitasatospora aureofaciens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
phosphoenolpyruvate + D-erythrose 4-phosphate + H2O | Kitasatospora aureofaciens | first enzyme of the aromatic amino acid biosynthesis | 2-dehydro-3-deoxy-D-arabino-heptonate 7-phosphate + phosphate | - |
? | |
phosphoenolpyruvate + D-erythrose 4-phosphate + H2O | Kitasatospora aureofaciens Tu24 | first enzyme of the aromatic amino acid biosynthesis | 2-dehydro-3-deoxy-D-arabino-heptonate 7-phosphate + phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Kitasatospora aureofaciens | - |
strain Tü 24 | - |
Kitasatospora aureofaciens Tu24 | - |
strain Tü 24 | - |
Purification (Comment) | Organism |
---|---|
170fold | Kitasatospora aureofaciens |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
phosphoenolpyruvate + D-erythrose 4-phosphate + H2O = 3-deoxy-D-arabino-hept-2-ulosonate 7-phosphate + phosphate | ordered ping-pong bi-bi mechanism | Kitasatospora aureofaciens | |
phosphoenolpyruvate + D-erythrose 4-phosphate + H2O = 3-deoxy-D-arabino-hept-2-ulosonate 7-phosphate + phosphate | first substrate is phosphoenolpyruvate | Kitasatospora aureofaciens |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1.3 | - |
partially purified enzyme | Kitasatospora aureofaciens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
phosphoenolpyruvate + D-erythrose 4-phosphate + H2O | - |
Kitasatospora aureofaciens | 2-dehydro-3-deoxy-D-arabino-heptonate 7-phosphate + phosphate | - |
? | |
phosphoenolpyruvate + D-erythrose 4-phosphate + H2O | first enzyme of the aromatic amino acid biosynthesis | Kitasatospora aureofaciens | 2-dehydro-3-deoxy-D-arabino-heptonate 7-phosphate + phosphate | - |
? | |
phosphoenolpyruvate + D-erythrose 4-phosphate + H2O | - |
Kitasatospora aureofaciens Tu24 | 2-dehydro-3-deoxy-D-arabino-heptonate 7-phosphate + phosphate | - |
? | |
phosphoenolpyruvate + D-erythrose 4-phosphate + H2O | first enzyme of the aromatic amino acid biosynthesis | Kitasatospora aureofaciens Tu24 | 2-dehydro-3-deoxy-D-arabino-heptonate 7-phosphate + phosphate | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Kitasatospora aureofaciens |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
phosphoenolpyruvate protects against inactivation | Kitasatospora aureofaciens |
additional information | - |
D-erythrose 4-phosphate, erythrose and ribose 5-phosphate increase rate of inactivation | Kitasatospora aureofaciens |
30 | - |
pH 6.0-8.0, stable for at least 2 h | Kitasatospora aureofaciens |
50 | - |
inactivation above | Kitasatospora aureofaciens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
- |
Kitasatospora aureofaciens |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.2 | 7.9 | pH 6.2: about 35% of maximal activity, pH 7.9: about 55% of maximal activity | Kitasatospora aureofaciens |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
6 | 8 | 30°C, stable for at least 2 h | Kitasatospora aureofaciens |
7 | 7.5 | maximal stability | Kitasatospora aureofaciens |